The Molecular Mechanisms Underlying Hidden Phenotypic Variation among Metallo-β-Lactamases
| Autor: | Nobuhiko Tokuriki, Raymond D. Socha, John Z Chen |
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| Rok vydání: | 2019 |
| Předmět: |
Models
Molecular Gene Transfer Horizontal Chromosomal translocation Biology beta-Lactamases 03 medical and health sciences 0302 clinical medicine Bacterial Proteins Genetic drift Structural Biology Transcription (biology) Genetic variation Escherichia coli Molecular Biology Gene 030304 developmental biology Genetics 0303 health sciences Bacteria Periplasmic space Phenotype Klebsiella pneumoniae Biological Variation Population Pseudomonas aeruginosa Horizontal gene transfer 030217 neurology & neurosurgery |
| Zdroj: | Journal of Molecular Biology. 431:1172-1185 |
| ISSN: | 0022-2836 |
| DOI: | 10.1016/j.jmb.2019.01.041 |
| Popis: | Genetic variation among orthologous genes has been largely formed through neutral genetic drift while maintaining the functional role of these genes. However, because the evolution of gene occurs in the context of each host organism, their sequence changes are also associated with adaptation to a specific environment. Thus, genetic variation can create critical phenotypic variation, particularly when genes are transferred to a new host by horizontal gene transfer. Unveiling “hidden phenotypic variation” is particularly important for genes that confer resistance to antibiotics. However, our understanding of the molecular mechanisms that underlie phenotypic variation remains limited. Here we sought to determine the extent of phenotypic variation in the B1 metallo-β-lactamase (MBL) family and its molecular basis by systematically characterizing eight MBL orthologs, including NDM-1 and VIM-2 and IMP-1. We found that these MBLs confer diverse levels of resistance. The phenotypic variation cannot be explained by variation in catalytic efficiency alone; rather, it is the combination of the catalytic efficiency and abundance of functional periplasmic enzyme that best predicts the observed variation in resistance. The level of functional periplasmic expression varied dramatically between MBL orthologs. This was the result of changes at multiple levels of each ortholog's: (1) quantity of mRNA, (2) amount of MBL expressed, and (3) efficacy of functional enzyme translocation to the periplasm. Overall, it is the interaction between each gene and the host's underlying cellular processes (transcription, translation, and translocation) that determines MBL genetic incompatibility through horizontal gene transfer. These host-specific processes may constrain the effective spread and deployment of MBLs to certain host species and could explain the current observed distribution bias. |
| Databáze: | OpenAIRE |
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