Effects of dodder total flavone on polycystic ovary syndrome rat models induced by DHEA combined HCG
| Autor: | Mengyan Li, Xiaoli Yan, Mengfan Peng, Mingsan Miao, Zhenzhen Wei, Zheng-Wang Zhu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine endocrine system medicine.medical_specialty Uterus Dehydroepiandrosterone Ovary 01 natural sciences Article Human chorionic gonadotropin 03 medical and health sciences Internal medicine medicine Vaginal smear lcsh:QH301-705.5 business.industry Polycystic ovary 030104 developmental biology medicine.anatomical_structure Endocrinology lcsh:Biology (General) Immunohistochemistry General Agricultural and Biological Sciences Pancreas business hormones hormone substitutes and hormone antagonists 010606 plant biology & botany |
| Zdroj: | Saudi Journal of Biological Sciences, Vol 26, Iss 4, Pp 821-827 (2019) |
| ISSN: | 1319-562X |
| DOI: | 10.1016/j.sjbs.2019.02.007 |
| Popis: | Aims: Explore the effects of dodder total flavone on polycystic ovary syndrome (PCOS) rat models induced by dehydroepiandrosterone (DHEA) combined human chorionic gonadotropin (HCG). Methods: Except the blank group, the rest of the rats were injected with DHEA 6 mg/100 g on the back of the neck and 1.5 IU HCG each day, for 21 consecutive days. On the 16th day of modeling, vaginal smear was performed to select the model rats, which were randomly divided into model group, dacin-35 group, large, middle and small dose dodder total flavonoids groups, and given the medicine for three weeks. At the end of the last administration, take samples, so as to calculate the ovaries and uterus indexes, measure serum LH/FSH ratio, P, PRL and INS levels, fixed the uterus and pancreas in 10% formalin solution and stained with HE to observe the morphological changes of the organs. And measure the expression of TNF-α and IGF-l proteins in ovaries by immunohistochemistry. Results: Compared with the blank group, ovarian and uterine indexes, serum LH/FSH ratio, serum PRL and INS levels, ovary TNF-α and IGF-l protein expression were significantly increased, and significant pathological changes were observed in the uterine and pancreatic tissues in model group (P |
| Databáze: | OpenAIRE |
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