Arsenic stress activates MAP kinase in rice roots and leaves
| Autor: | Gubbala Vani, Kundan Kumar, Kudupudi Prabhakara Rao, Meetu Gupta, Mohit Misra, Alok Krishna Sinha, Dhammaprakash Pandhari Wankhede |
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| Rok vydání: | 2011 |
| Předmět: |
Models
Molecular MAPK/ERK pathway Arsenites MAP Kinase Signaling System Biophysics Biology Nitric Oxide Plant Roots Biochemistry Arsenic Nitric oxide chemistry.chemical_compound Gene family Protein Interaction Domains and Motifs RNA Messenger Molecular Biology Arsenite Mitogen-Activated Protein Kinase Kinases chemistry.chemical_classification Reactive oxygen species Arsenic toxicity food and beverages Oryza biology.organism_classification Cell biology Enzyme Activation Plant Leaves Oxidative Stress chemistry RNA Plant Seedling Multiprotein Complexes Mitogen-activated protein kinase biology.protein Mitogen-Activated Protein Kinases Reactive Oxygen Species |
| Zdroj: | Archives of Biochemistry and Biophysics. 506:73-82 |
| ISSN: | 0003-9861 |
| DOI: | 10.1016/j.abb.2010.11.006 |
| Popis: | The toxic metalloid arsenite has become a potential threat to rice growing regions leading to serious contamination in food chain. In the present study effect of different physiological concentration of arsenite that is toxic and triggers the molecular events were evaluated in rice seedlings. Along with severe effect on the growth of rice seedling, production of reactive oxygen species (ROS) and nitric oxide (NO) in arsenite treated rice roots was also observed. Activation of a 42kDa mitogen activated protein kinase (MAPK/MPK) by arsenite was observed in rice leaves and 42 and 44kDa in roots in dose dependent manner. The activated MAPK could be immunoprecipitated with anti-phospho-tyrosine antibody, 4G10. The kinetic of MAPK activation by arsenite was found to be dose dependent. Transcript analysis of MAPK family and immunokinase assay in arsenite treated rice seedling revealed significant level of induction in OsMPK3 transcripts in leaves and OsMPK3, OsMPK4 transcripts in roots. Among MAPK kinase (MKKs) gene family, OsMKK4 transcripts were found to be induced in arsenite treated rice leaves and roots. In-silico homology modeling and docking analysis supported OsMPK3-OsMKK4 interaction. The data indicates that arsenite stress is transduced through MAPK signaling cascade in rice. |
| Databáze: | OpenAIRE |
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