Impact of Physicochemical Character of Hydrophobic Porous Carrier on Reactivity of Immobilized Lipase Progressing Toward Higher Reaction Rate and High Yield in Repeated Use
| Autor: | Masanao Imai, Masakazu Naya |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Hydrophobic porous carrier
Chromatography Materials science biology General Medicine biology.organism_classification Candida rugosa Reaction rate chemistry.chemical_compound chemistry Immobilized lipase Yield (chemistry) biology.protein Reactivity (chemistry) Glutaraldehyde Rhizopus arrhizus Particle size Lipase Engineering(all) Cross-linking |
| Zdroj: | Procedia Engineering. :1004-1015 |
| ISSN: | 1877-7058 |
| DOI: | 10.1016/j.proeng.2012.07.492 |
| Popis: | Impact of physicochemical character of hydrophobic porous carrier on reactivity of immobilized lipase from Candida rugosa, Rhizopus arrhizus , and Wheat Germ was investigated by Accurel MP100 carrier employed. Accurel MP100 was made of a polypropylene-based hydrophobic porous support. Lipase was adsorbed and immobilized on the Accurel pores with the aid of cross-linking agent glutaraldehyde. Immobilized yield attended satisfactorily high level (98%). Effectiveness factor of immobilized lipase was improved to use reduced size Accurel particles. In the case of Candida rugosa used (particle size 500-840 μm), the effectiveness factor exceeded 0.5. Suitable carrier size to realize sufficient high reaction rate was practically depended on lipase species used. Repeated use of immobilized lipase was attractively performed and stable. The productivity was remained almost 50%. Accurel MP100 was expected as hydrophobic friendly lipase carrier progressing toward higher reaction rate and high yield in repeated use. |
| Databáze: | OpenAIRE |
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