Expression of ras proto-oncogenes in the Dunning R3327 rat prostatic adenocarcinoma system
| Autor: | D. B. Cooke, Don D. Mickey, Channing J. Der, Peter Petrusz, J. T. Isaacs, Valerie E. Quarmby, Frank S. French |
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| Rok vydání: | 1988 |
| Předmět: |
Male
Pathology medicine.medical_specialty medicine.drug_class Urology Immunocytochemistry Biology Adenocarcinoma urologic and male genital diseases Proto-Oncogene Proteins p21(ras) Prostate Proto-Oncogene Proteins medicine Tumor Cells Cultured Animals RNA Messenger Messenger RNA Prostatic Neoplasms Transfection medicine.disease Androgen Phenotype Rats medicine.anatomical_structure Genes ras Oncology Tumor progression Cancer research |
| Zdroj: | The Prostate. 13(4) |
| ISSN: | 0270-4137 |
| Popis: | Steady-state levels of c-Ha-ras mRNA were measured in eight sublines of the Dunning R3327 rat prostatic adenocarcinoma. As a control, normal dorsal prostate tissue was studied. Increased expression of c-Ha-ras is associated with tumor progression in one lineage of the Dunning R3327 system (H to AT1 to MAT-Lu and MAT-Ly-Lu). Here ras mRNA increases as the tumor advances from androgen dependence and a high degree of differentiation to an anaplastic aneuploid phenotype with high metastatic potential. However, in the other Dunning lineage (H to HI to HI-F to AT3), expression of c-Ha-ras is variable and does not correlate with tumor progression. Immunocytochemistry showed that levels of the c-Ha-ras p21 protein paralleled steady-state mRNA levels in all variants. Transfection assays, using NIH/3T3 cells, suggested that the ras loci were not activated in the R3327 tumors. Levels of c-Ki-ras mRNA were also measured in the Dunning tumors; these did not correlate with tumor progression in either lineage. Expression of N-ras mRNA was not detected in the Dunning tumors. |
| Databáze: | OpenAIRE |
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