Gypenosides improves nonalcoholic fatty liver disease induced by high-fat diet induced through regulating LPS/TLR4 signaling pathway

Autor: Kungen Wang, Yihui Zhi, Huang Liquan, Shen Wei, Shen Shuhua
Rok vydání: 2020
Předmět:
Lipopolysaccharides
Male
0301 basic medicine
medicine.medical_specialty
Cell Survival
Biology
Diet
High-Fat
Transfection
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Non-alcoholic Fatty Liver Disease
Internal medicine
Lipid droplet
Nonalcoholic fatty liver disease
medicine
Animals
Humans
Oil Red O
Rats
Wistar
Molecular Biology
Cell Line
Transformed
Triglyceride
Plant Extracts
Fatty liver
Lipid metabolism
Cell Biology
medicine.disease
Gynostemma
Rats
Toll-Like Receptor 4
Disease Models
Animal
Treatment Outcome
030104 developmental biology
Endocrinology
chemistry
030220 oncology & carcinogenesis
Hepatocytes
TLR4
lipids (amino acids
peptides
and proteins)
Liver function
Research Paper
Drugs
Chinese Herbal
Phytotherapy
Signal Transduction
Developmental Biology
Zdroj: Cell Cycle
ISSN: 1551-4005
1538-4101
DOI: 10.1080/15384101.2020.1829800
Popis: Background The contents of lipopolysaccharide (LPS) and Toll-like receptor 4 (TLR4) are significantly increased during the progression of nonalcoholic fatty liver disease (NAFLD). The study investigated the role of the LPS/TLR4 signaling pathway in improving gypenosides (Gyp) on NAFLD. Methods NAFLD model were established in rats and treated by Gyp. Pathological changes of liver tissues were observed by Hematoxylin and Eosin (HE) staining. Lipid metabolism and insulin resistance were measured. Expressions of inflammatory factors and protein of LPS/TLR4 downstream pathway were detected by qRT-PCR and Western blotting. THLE-2 cells were treated by free-fatty acid (FFA), Gyp, and LPS, and then transfected with TLR4. Next, cell viability was detected by MTT. Lipid droplet deposition and Triglyceride (TG) content were determined by Oil Red O staining and ELISA. Results Gyp protected fatty liver tissues in NAFLD model, and significantly reversed cholesterol increased by high-fat diet. Moreover, Gyp increased SOD content and decreased the contents of AST, ALT, MDA, HSI, FBG, FINS, HOMA-IR, IL-1β, and TNF-α, and promoted the expressions of TLR4, LPS, MyD88, p-IκBα, and reduced the expressions of p-p65 and IκBα in the NAFLD model. Gyp treatment significantly reduced lipid droplet deposition, increased TG content and MyD88, p-IκBα, p-p65 in FFA-induced liver cells, but LPS and TLR4 greatly reversed improvement of FFA by Gyp. Conclusion Gypenosides could improve liver function, lipid metabolism, insulin resistance, and levels of inflammatory factors in NAFLD model by regulating LPS/TLR4 signaling pathway in vitro and in vivo.
Databáze: OpenAIRE
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