M2-like macrophage infiltration and transforming growth factor-β secretion during socket healing process in mice
| Autor: | Kanji Horibe, Hiroaki Nakamura, Miroku Hara |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male medicine.medical_treatment Bone healing 03 medical and health sciences Mice 0302 clinical medicine Osteogenesis Transforming Growth Factor beta medicine Macrophage Animals Tooth Socket General Dentistry Wound Healing Chemistry Growth factor Macrophages Osteoblast 030206 dentistry Cell Biology General Medicine medicine.disease Cell biology RUNX2 030104 developmental biology medicine.anatomical_structure Otorhinolaryngology Tooth Extraction Cytokine secretion Infiltration (medical) Transforming growth factor |
| Zdroj: | Archives of oral biology. 123 |
| ISSN: | 1879-1506 |
| Popis: | Objective Macrophages are involved in tissue inflammation and repair through cytokine secretion. However, the contribution of macrophages to healing and osteogenesis after tooth extraction remains unclear. Therefore, we investigated the distribution of osteoblastic cells and macrophages in the early healing process after tooth extraction. Methods The maxillary first molars of 6-week-old male mice were extracted. The maxilla was collected 1, 3, and 7 days after extraction. The states of socket healing, localization of osteoblastic markers, and macrophage infiltration were sequentially observed by micro-CT imaging and immunohistochemistry. Results On day 3 after tooth extraction, α-smooth muscle actin (SMA)-positive cells, osteoprogenitor cells at fracture healing, were observed in the socket. Several α-SMA-positive cells also expressed Runx2, the early osteoblast differentiation marker. The infiltration of F4/80-positive, mature macrophages and CD206-positive, M2-like macrophages was noted in the socket. However, CD169-positive macrophages (Osteomac), which are involved in fracture healing, were not detected in the socket. F4/80-positive and CD206-positive macrophages also showed the localization of transforming growth factor-β (TGF-β), which promotes osteoprogenitor cell proliferation and early differentiation. Phosphorylated Smad3, a downstream mediator of the signal activity of TGF-β, was detected in α-SMA-positive cells. On day 7, the extracted socket contained a large amount of new bone. Tartrate-resistant acid phosphatase (TRAP)-positive osteoclasts were detected on bone surfaces. Conclusion Our data indicate that M2-like macrophages regulate the proliferation and differentiation of α-SMA-positive cells by secreting TGF-β at the early stage of socket healing, and also suggest the importance of macrophages in healing and bone formation after tooth extraction. |
| Databáze: | OpenAIRE |
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