Functional analysis of the engineered cardiac tissue grown on recombinant spidroin fiber meshes
| Autor: | Konstantin Agladze, K. V. Sidoruk, V. G. Bogush, Olga A. Agapova, Alexander S Teplenin, Igor I. Agapov, Anna Krasheninnikova, Nadezhda Agladze |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
lcsh:Medicine
law.invention Tissue engineering law Cell Adhesion Animals Myocyte Myocytes Cardiac Cell adhesion lcsh:Science Cells Cultured Cell Proliferation Multidisciplinary Tissue Engineering Tissue Scaffolds biology Spidroin Chemistry lcsh:R Adhesion Molecular biology Recombinant Proteins Rats Cell biology Fibronectin biology.protein Recombinant DNA lcsh:Q Fibroins Immunostaining Research Article |
| Zdroj: | PLoS ONE, Vol 10, Iss 3, p e0121155 (2015) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | In the present study, we examined the ability of the recombinant spidroin to serve as a substrate for the cardiac tissue engineering. For this purpose, isolated neonatal rat cardiomyocytes were seeded on the electrospun spidroin fiber matrices and cultured to form the confluent cardiac monolayers. Besides the adhesion assay and immunostaining analysis, we tested the ability of the cultured cardiomyocytes to form a functional cardiac syncytium by studying excitation propagation in the cultured tissue with the aid of optical mapping. It was demonstrated that recombinant spidroin fiber meshes are directly suitable for the adherence and growth of the cardiomyocytes without additional coating with the attachment factors, such as fibronectin. |
| Databáze: | OpenAIRE |
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