Automated quantitative determination of a new polyamine biosynthesis inhibitor (CGP 48 664) and a potential metabolite in human and animal plasma by high-performance liquid chromatography
| Autor: | P. Zbinden, P.H. Degen |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Adenosylmethionine Decarboxylase
Metabolite Amidines Guanidines Sensitivity and Specificity High-performance liquid chromatography chemistry.chemical_compound Dogs Drug Stability Blood plasma Animals Humans Solid phase extraction Enzyme Inhibitors Chromatography High Pressure Liquid Structural analog Autoanalysis Chromatography biology Chemistry Extraction (chemistry) Hydrazones General Chemistry Rats Enzyme inhibitor Indans biology.protein Regression Analysis Quantitative analysis (chemistry) |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 681:339-345 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/0378-4347(96)00026-6 |
| Popis: | A specific and sensitive liquid chromatographic assay for the determination of 4-amidino-1-indanone-2'-amidinohydrazone (CGP 48 664, I) and a potential metabolite, 2-(4-carbamoyl-2,3-dihydro-1H-inden-1-yliden) hydrazine carboximidamide (CGP 53 391, II), in human and animal plasma was developed. CGP 51 467, a structural analog, was added to the plasma samples (up to 1 ml) as an internal standard. After mixing, the samples were processed automatically using an ASPEC solid-phase extraction system. The final extracts were chromatographed on a 5 microns Purospher RP-18 HPLC column. Chromatography was performed using a gradient system and UV detection. The described HPLC method is suitable for specific and quantitative measurement of concentrations of I, as well as its potential metabolite II down to 5-10 ng/ml in human and animal (dog, rat) plasma with acceptable reproducibility and accuracy. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|