Evaluation ofin vitroandin vivogenotoxic and antigenotoxic effects ofRhus coriaria
| Autor: | Taygun Timocin, Mehmet Arslan, Hasan Basri Ila |
|---|---|
| Přispěvatelé: | Çukurova Üniversitesi, 0-Belirlenecek |
| Rok vydání: | 2019 |
| Předmět: |
Health
Toxicology and Mutagenesis antigenotoxicity 010501 environmental sciences Toxicology medicine.disease_cause 01 natural sciences 03 medical and health sciences 0302 clinical medicine In vivo plasmid Coriaria medicine Oxidative Sstress oxidative stress Cytotoxicity 0105 earth and related environmental sciences Pharmacology Chemical Health and Safety Traditional medicine biology Chemistry genotoxicity Public Health Environmental and Occupational Health General Medicine biology.organism_classification In vitro Rhus coriaria cytotoxicity 030217 neurology & neurosurgery Genotoxicity Oxidative stress |
| Zdroj: | Drug and Chemical Toxicology. 44:409-417 |
| ISSN: | 1525-6014 0148-0545 |
| Popis: | Rhus coriaria has been important in the treatment of many diseases in traditional use. In this content, the genotoxic, antigenotoxic, and oxidative stress effects of methanol extract of R. coriaria (RCE) were investigated in this study. Two hundred fifty, 500, or 750 µg/mL concentrations of RCE were not found to have DNA damaging effect on pET22-b(+) plasmid and were unable to induce micronuclei in human lymphocytes (24 or 48 h treatment period). However, it did not inhibit the genotoxic effect of mitomycin-c (0.25 µg/mL). Cytotoxic effects of RCE were investigated using mitotic index (MI) and nuclear division index (NDI). Five hundred, 1000, and 2000 mg/kg concentrations of RCE did not induce chromosome aberrations in rat bone marrow cells for 12 or 24 h treatment period. In addition, 2000 mg/kg concentration of RCE showed an antigenotoxic effect by decreasing to genotoxic effect of 400 mg/kg urethane at 12 and 24 h treatment periods. RCE showed cytotoxic effects by significantly decreasing NDI. Moreover, RCE increased cytotoxic effect of Mitomycin C (MMC). However, RCE did not induce cytotoxicity in rat bone marrow cells. The highest concentration of RCE reduced total oxidant level in 12 h treatment. Interestingly, the lowest total oxidant level was found in rats blood treated with the lowest concentration RCE and urethane together. Thousand and 2000 mg/kg concentrations of RCE decreased total antioxidant levels of rat blood at 24 h treatment period. Our results showed that RCE possess cytotoxic effect in short-term treatments in vitro. However, it does not demonstrate genotoxic or cytotoxic effects in vivo. © 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|