Expression of bcl-2 protein and Ki-67 nuclear proliferation antigen in benign and malignant cutaneous T-cell infiltrates
| Autor: | Jennifer W. Gould, Bruce R. Smoller, Andreas C. Haeffner, R. Dummer, Roger A. Warnke, Sara A. Michie, Gary S. Wood, Donna L. Kell |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Pathology
medicine.medical_specialty Skin Neoplasms Histology Discoid lupus erythematosus Lymphoid Tissue Dermatology Lymphoma T-Cell Pathology and Forensic Medicine Mycosis Fungoides Lymphomatoid Papulosis Proto-Oncogene Proteins Humans Medicine Lymphomatoid papulosis Mycosis fungoides Hyperplasia biology business.industry Mantle zone Nuclear Proteins medicine.disease Peripheral T-cell lymphoma Neoplasm Proteins Ki-67 Antigen Proto-Oncogene Proteins c-bcl-2 Ki-67 biology.protein Cutaneous lymphoid hyperplasia business |
| Zdroj: | Journal of Cutaneous Pathology. 22:11-17 |
| ISSN: | 1600-0560 0303-6987 |
| DOI: | 10.1111/j.1600-0560.1995.tb00733.x |
| Popis: | The bcl-2 protein prolongs cell life by inhibiting apoptosis. Its expression has been studied in a variety of normal tissues and lymphomas but there is minimal information available concerning bcl-2 expression by benign and malignant cutaneous T-cells. Therefore, we investigated bcl-2 expression in a wide variety of cutaneous T-cell infiltrates using one- and two-color immunohistologic techniques. bcl-2 was expressed by the majority of lesional CD3+ T-cells in most cases. This included 22/26 cases of mycosis fungoides (MF), 3/3 cases of non-MF cutaneous T-cell lymphoma, 5/5 cases of lymphomatoid papulosis, 4/4 cases of T-cell rich cutaneous lymphoid hyperplasia, 2/3 cases of bullous pemphigoid, 2/2 cases of discoid lupus erythematosus and 1/1 case of lichen planus. Titration experiments and comparative studies of tonsil section positive controls revealed that, relative to mantle zone B-cells, there was over- expression of bcl-2 by a variable subset of T-cells in most cases. Assessment of multiple biopsies in a subset of MF cases showed stable expression of bcl-2 over intervals of up to two years. In contrast to the widespread expression of bcl-2 in both early and advanced MF skin lesions, abundant expression of the nuclear proliferation antigen, Ki-67, was skewed toward advanced MF skin lesions. Ten percent or more Ki-67+ cells were present in 5% of patients with patches/thin plaques, 38% with moderate plaques, 64% with thick plaques and 100% with tumor nodules.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text