Quantitative comparison of cancer and normal cell adhesion using organosilane monolayer templates: an experimental study on the anti-adhesion effect of green-tea catechins
| Autor: | Rumi Sakamoto, Yuko Sato, Hiroshi Yoshikawa, Kentaro Masuda, Hideaki Yamamoto, Takashi Tanii, Yuko Takeuchi, Kosaku Ito, Seiichiro Nakabayashi, Kentaro Iketaki, Takahisa Matsuzaki, Eisuke Kakinuma |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell complex mixtures Catechin 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cell Line Tumor Cell Adhesion medicine Humans Cell adhesion Pancreas Tea Chemistry food and beverages Cell Biology General Medicine Adhesion Embryonic stem cell Pancreatic Neoplasms 030104 developmental biology medicine.anatomical_structure Biochemistry Cell culture 030220 oncology & carcinogenesis Cancer cell Biophysics Stem cell Developmental Biology |
| Zdroj: | In Vitro Cellular & Developmental Biology - Animal. 52:799-805 |
| ISSN: | 1543-706X 1071-2690 |
| DOI: | 10.1007/s11626-016-0049-6 |
| Popis: | The main constituent of green tea, (-)-Epigallocatechin-3-O-gallate (EGCG), is known to have cancer-specific chemopreventive effects. In the present work, we investigated how EGCG suppresses cell adhesion by comparing the adhesion of human pancreatic cancer cells (AsPC-1 and BxPC-3) and their counterpart, normal human embryonic pancreas-derived cells (1C3D3), in catechin-containing media using organosilane monolayer templates (OMTs). The purpose of this work is (1) to evaluate the quantitativeness in the measurement of cell adhesion with the OMT and (2) to show how green-tea catechins suppress cell adhesion in a cancer-specific manner. For the first purpose, the adhesion of cancer and normal cells was compared using the OMT. The cell adhesion in different type of catechins such as EGCG, (-)-Epicatechin-3-O-gallate (ECG) and (-)-Epicatechin (EC) was also evaluated. The measurements revealed that the anti-adhesion effect of green-tea catechins is cancer-specific, and the order is EGCG≫ECG>EC. The results agree well with the data reported to date, showing the quantitativeness of the new method. For the second purpose, the contact area of cells on the OMT was measured by reflection interference contrast microscopy. The cell-OMT contact area of cancer cells decreases with increasing EGCG concentration, whereas that of normal cells remains constant. The results reveal a twofold action of EGCG on cancer cell adhesion-suppressing cell attachment to a candidate adhesion site and decreasing the contact area of the cells-and validates the use of OMT as a tool for screening cancer cell adhesion. |
| Databáze: | OpenAIRE |
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