SEMA3A signaling controls layer-specific interneuron branching in the cerebellum
| Autor: | Jean-Michel Cioni, Carole Jourdan, Andrea B. Huber, Josh Huang, Véronique Saywell, Ludovic Telley, Fabrice Ango, Céline Jahannault-Talignani, Christelle Cadilhac |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
animal structures
Biology Axon hillock General Biochemistry Genetics and Molecular Biology Mice FYN Axon terminal Interneurons Basket cell Cerebellum medicine Biological neural network Animals Telodendron Axon Cyclic GMP gamma-Aminobutyric Acid Mice Knockout Agricultural and Biological Sciences(all) Biochemistry Genetics and Molecular Biology(all) Semaphorin-3A Axons Cell biology Protein Transport medicine.anatomical_structure Biochemistry nervous system Guanylate Cyclase Axon guidance General Agricultural and Biological Sciences Signal Transduction |
| Zdroj: | Curr. Biol. 23, 850-861 (2013) |
| Popis: | Summary Background GABAergic interneurons regulate the balance and dynamics of neural circuits, in part, by elaborating their strategically placed axon branches that innervate specific cellular and subcellular targets. However, the molecular mechanisms that regulate target-directed GABAergic axon branching are not well understood. Results Here we show that the secreted axon guidance molecule, SEMA3A, expressed locally by Purkinje cells, regulates cerebellar basket cell axon branching through its cognate receptor Neuropilin-1 (NRP1). SEMA3A was specifically localized and enriched in the Purkinje cell layer (PCL). In sema3A −/− and nrp1 sema−/sema− mice lacking SEMA3A-binding domains, basket axon branching in PCL was reduced. We demonstrate that SEMA3A-induced axon branching was dependent on local recruitment of soluble guanylyl cyclase (sGC) to the plasma membrane of basket cells, and sGC subcellular trafficking was regulated by the Src kinase FYN. In fyn -deficient mice, basket axon terminal branching was reduced in PCL, but not in the molecular layer. Conclusions These results demonstrate a critical role of local SEMA3A signaling in layer-specific axonal branching, which contributes to target innervation. |
| Databáze: | OpenAIRE |
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