Altered levels of the Taraxacum kok-saghyz (Russian dandelion) small rubber particle protein, TkSRPP3, result in qualitative and quantitative changes in rubber metabolism
| Autor: | David K. Shintani, Maureen C. Whalen, Amanda Skaggs, Deborah J. Scott, Upul Hathwaik, Aise Taban Nural, Katrina Cornish, Rebekah Woolsey, Jillian Collins-Silva, Kathleen M. Schegg, Colleen M. McMahan |
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| Rok vydání: | 2011 |
| Předmět: |
Time Factors
Taraxacum Dandelion Plant Science Horticulture complex mixtures Biochemistry Plant Roots chemistry.chemical_compound Natural rubber Biosynthesis RNA interference Gene Expression Regulation Plant Botany Taraxacum kok-saghyz RNA Messenger Molecular Biology Plant Proteins chemistry.chemical_classification biology technology industry and agriculture General Medicine Polymer biology.organism_classification Plants Genetically Modified body regions chemistry visual_art visual_art.visual_art_medium RNA Interference Hevea brasiliensis Rubber psychological phenomena and processes Function (biology) |
| Zdroj: | Phytochemistry. 79 |
| ISSN: | 1873-3700 |
| Popis: | Several proteins have been identified and implicated in natural rubber biosynthesis, one of which, the small rubber particle protein (SRPP), was originally identified in Hevea brasiliensis as an abundant protein associated with cytosolic vesicles known as rubber particles. While previous in vitro studies suggest that SRPP plays a role in rubber biosynthesis, in vivo evidence is lacking to support this hypothesis. To address this issue, a transgene approach was taken in Taraxacum kok-saghyz (Russian dandelion or Tk) to determine if altered SRPP levels would influence rubber biosynthesis. Three dandelion SRPPs were found to be highly abundant on dandelion rubber particles. The most abundant particle associated SRPP, TkSRPP3, showed temporal and spatial patterns of expression consistent with patterns of natural rubber accumulation in dandelion. To confirm its role in rubber biosynthesis, TkSRPP3 expression was altered in Russian dandelion using over-expression and RNAi methods. While TkSRPP3 over-expressing lines had slightly higher levels of rubber in their roots, relative to the control, TkSRPP3 RNAi lines showed significant decreases in root rubber content and produced dramatically lower molecular weight rubber than the control line. Not only do results here provide in vivo evidence of TkSRPP proteins affecting the amount of rubber in dandelion root, but they also suggest a function in regulating the molecular weight of the cis-1, 4-polyisoprene polymer. |
| Databáze: | OpenAIRE |
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