Melatonin regulates the ovarian function and enhances follicle growth in aging laying hens via activating the mammalian target of rapamycin pathway
| Autor: | Rongyan Zhou, Hui Chen, Ren-Lu Huang, De-He Wang, Yi-Fan Chen, Chen-Xuan Huang, Hao Erying, Ya-Guang Tong |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
Physiology and Reproduction media_common.quotation_subject melatonin Ovary Biology hen Antioxidants follicle Avian Proteins Superoxide dismutase Melatonin Random Allocation Follicle Ovarian Follicle Internal medicine Follicular phase medicine Animals Ovulation PI3K/AKT/mTOR pathway lcsh:SF1-1100 media_common Cell growth TOR Serine-Threonine Kinases aging General Medicine Endocrinology medicine.anatomical_structure mTOR biology.protein Female Animal Science and Zoology lcsh:Animal culture Chickens Injections Intraperitoneal Signal Transduction medicine.drug |
| Zdroj: | Poultry Science, Vol 99, Iss 4, Pp 2185-2195 (2020) Poultry Science |
| ISSN: | 0032-5791 |
| DOI: | 10.1016/j.psj.2019.11.040 |
| Popis: | The signal pathway of target of rapamycin (TOR) plays an important role in regulating cell growth and proliferation, follicular development, and ovulation. Melatonin (N-acetyl-5-methoxytryptamine) (MT) is involved in the regulation of many physiological functions in animals. Recent studies have shown that MT affects the number and the degree of maturation of follicles in the ovary, but there are few studies concerning its mechanism. Therefore, the aim of this study was to investigate the mechanism of TOR signal pathway in the regulation of ovarian function by MT in aging laying hens. In the present study, a total of 60 hens (70-week-old) were randomly divided into 2 groups: control group and melatonin group (M). Melatonin was administered intraperitoneally at a dose of 20 mg/kg/D for 28 D in the M group. The results showed that MT significantly increased the levels of the antioxidant enzymes superoxide dismutase and total antioxidant capacity (P < 0.01) as well as levels of immunoglobulin (IgA, IgG, and IgM) (P < 0.05) and the reproductive hormones estradiol and luteinizing hormone (P < 0.01) in the plasma and also increased the numbers of middle white follicles and small white follicles (P < 0.05) and decreased the level of reactive oxygen species in plasma (P < 0.01) in laying hens. There were higher expression levels in MT receptor A (P < 0.05), melatonin receptor B (P < 0.01), and tuberous sclerosis complex 2 (P < 0.01). Activation of TOR, 4E binding protein-l (4E-BP1), and ribosomal protein 6 kinase (P |
| Databáze: | OpenAIRE |
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