Specific Antibodies for Immunochemical Detection of Wood-Derived Hemicelluloses

Autor:	Arja Lappalainen, Maija Tenkanen, Jaakko Pere
Rok vydání:	2003
Předmět:	Specific antibody Biochemistry Chemistry food and beverages
Zdroj:	ACS Symposium Series ISBN: 9780841238428 Hemicelluloses: Science and Technology University of Helsinki
DOI:	10.1021/bk-2004-0864.ch010
Popis:	Antibodies raised against wood-derived hemicelluloses can be used for chemical mapping of pulp fibres. Polyclonal antibodies were produced against cell wall carbohydrates, i.e. xylan, mannan and pectin, using both oligosaccharides coupled to BSA and polymeric carbohydrates as antigens. The antisera were checked for their specificity using ELISA techniques. In general, oligosaccharide-BSA conjugates were more antigenic and established higher affinities against the tested antigens as compared with the corresponding polymeric hemicelluloses. Applicability of the antisera was further tested on pulp fibres of known chemical composition. Labelling patterns of pulp fibres were in agreement with available information on the surface chemistry of fibres. FITC conjugate was shown to be suitable for delignified samples whereas AlexaFluor 633 label can also be used on lignin-containing material.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b17ad6e282c91466890e45089607dacb https://doi.org/10.1021/bk-2004-0864.ch010 Zobrazit plný text záznamu