Production of glucose-fructose oxidoreductase and ethanol by Zymomonas mobilis ATCC 29191 in medium containing corn steep liquor as a source of vitamins
| Autor: | Elisabeth Wisbeck, Hoch I, Mauricio Moura da Silveira, Rainer Jonas |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Zymomonas
Ethanol biology General Medicine Vitamins biology.organism_classification Applied Microbiology and Biotechnology Corn steep liquor Zymomonas mobilis Gluconates Culture Media chemistry.chemical_compound Biochemistry chemistry Glucose-fructose oxidoreductase Gluconic acid Yeast extract Sorbitol Ethanol fuel Food science Oxidoreductases Biotechnology |
| Zdroj: | Applied microbiology and biotechnology. 55(4) |
| ISSN: | 0175-7598 |
| Popis: | Different concentrations of corn steep liquor (CSL) were tested in the cultivation of Zymomonas mobilis. Cell growth, ethanol production, and the formation of glucose-fructose oxidoreductase (GFOR) and glucono-delta-lactonase (GL), the enzymes responsible for the bio-production of gluconic acid and sorbitol, were examined. The cell yields using 25 g CSL l(-1) and 40 g CSL l(-1) (Y(X,S) approximately 0.031 g g(-1)) were close to that obtained with 5 g yeast extract (YE) l(-1). With 5 g CSL l(-1) and 15 g CSL l(-1), the nutritional limitation led to smaller Y(X/S). Using 100 g CSL l(-1) produced an inhibitory effect on cell growth. Similar ethanol yields (92-95%) were calculated for each concentration of CSL and also for YE medium. The highest specific GFOR/GL activities (13.2-13.5 U g(-1) dry cell) were reached with 25 g CSL l(-1) and 40 g CSL l(-1), values comparable to that achieved with 5 g YE l(-1). The results confirm that CSL is an effective and cheap supplement for Z. mobilis medium, increasing the economic potential of a large-scale bio-production of sorbitol and gluconic acid by untreated Z. mobilis cells. The economic feasibility of the process is discussed. |
| Databáze: | OpenAIRE |
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