Endometrial microbiota in infertile women with and without chronic endometritis as diagnosed using a quantitative and reference range-based method
| Autor: | Jacqueline Pui Wah Chung, Tracy Sze Man Law, Karen Ka-Wing Wong, Stephen Kwok-Wing Tsui, Elaine Yee-Ling Ko, Yingyu Liu, Wing-Ching Cheung, Tin-Chiu Li, Xiaoyan Chen, Stephen S.C. Chim |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Reference range Gastroenterology Endometrium 03 medical and health sciences 0302 clinical medicine Reference Values Internal medicine Lactobacillus Biopsy medicine Humans 030219 obstetrics & reproductive medicine Bacteria Lactobacillus crispatus biology medicine.diagnostic_test business.industry Microbiota Anaerococcus Obstetrics and Gynecology General Medicine biology.organism_classification 030104 developmental biology Reproductive Medicine Case-Control Studies Chronic Disease Dialister Female Endometritis Chronic Endometritis business Infertility Female Endometrial biopsy |
| Zdroj: | Fertility and Sterility. 112:707-717.e1 |
| ISSN: | 0015-0282 |
| DOI: | 10.1016/j.fertnstert.2019.05.015 |
| Popis: | Objective To systematically compare the endometrial microbiota in infertile women with and without chronic endometritis (CE), as diagnosed by a quantitative and reference range-based method. Design Case-control observational study. Setting University-affiliated hospital. Patient(s) One hundred and thirty infertile women. Intervention(s) Endometrial biopsy and fluid (uterine lavage, UL) collected precisely 7 days after LH surge, with plasma cell density (PCD) determined based on Syndecan-1 (CD138)-positive cells in the entire biopsy section and culture-independent massively parallel sequencing of the 16S ribosomal RNA gene performed on both the CE and non-CE endometrial fluid samples. Main Outcome Measure(s) Relative abundance of bacterial taxa. Result(s) Chronic endometritis was diagnosed if the PCD was above the 95th percentile (>5.15 cells per 10 mm2) of the reference range in fertile control subjects. With this stringent diagnostic criterion, 12 women (9%) were diagnosed with CE. Sequencing was successfully performed on all endometrial samples obtained by UL) (CE, n = 12; non-CE, n = 118). The median relative abundance of Lactobacillus was 1.89% and 80.7% in the CE and non-CE microbiotas, respectively. Lactobacillus crispatus was less abundant in the CE microbiota (fold-change, range: 2.10–2.30). Eighteen non-Lactobacillus taxa including Dialister, Bifidobacterium, Prevotella, Gardnerella, and Anaerococcus were more abundant in the CE microbiota (fold-change, 2.10–18.9). Of these, Anaerococcus and Gardnerella were negatively correlated in relative abundance with Lactobacillus (SparCC correlation magnitude, range: 0.142–0.177). Conclusion(s) Chronic endometritis was associated with a statistically significantly higher abundance of 18 bacterial taxa in the endometrial cavity. Clinical Trials Registry Number ChiCTR-IOC-16007882. |
| Databáze: | OpenAIRE |
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