Confirming Hypoderma tarandi (Diptera: Oestridae) human ophthalmomyiasis by larval DNA barcoding
| Autor: | Arild Johnsen, Preben Ottesen, Bjørn Arne Rukke, Symira Cholidis |
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| Rok vydání: | 2013 |
| Předmět: |
Cytochrome C Oxidase Subunit 1
Male Eye Diseases ved/biology.organism_classification_rank.species Molecular Sequence Data Zoology Biology DNA barcoding DNA sequencing Hypoderma tarandi Electron Transport Complex IV Myiasis parasitic diseases Species identification Animals DNA Barcoding Taxonomic Humans Child Larva Ecology ved/biology Norway Diptera fungi Sequence Analysis DNA biology.organism_classification Dermatobia hominis Parasitology |
| Zdroj: | Acta parasitologica. 59(2) |
| ISSN: | 1896-1851 |
| Popis: | DNA barcoding is a practical tool for species identification, when morphological classification of an organism is difficult. Herein we describe the utilisation of this technique in a case of ophthalmomyiasis interna. A 12-year-old boy was infested during a summer holiday in northern Norway, while visiting an area populated with reindeer. Following medical examination, a Diptera larva was surgically removed from the boy’s eye and tentatively identified from its morphological traits as Hypoderma tarandi (L.) (Diptera: Oestridae). Ultimately, DNA barcoding confirmed this impression. The larval cytochrome c oxidase subunit 1 (COI) DNA sequence was matched with both profiles of five adult H. tarandi from the same region where the boy was infested, and other established profiles of H. tarandi in the Barcode of Life Data Systems (BOLD) identification engine. |
| Databáze: | OpenAIRE |
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