Analysis of blood parameters and molecular endometrial markers during early reperfusion in two ovine models of uterus transplantation
| Autor: | Barbara Hersant, Valérie Gelin, Laurent Galio, Mariam Raliou, François Vialard, Olivier Dubois, Anne Couturier-Tarrade, Corinne Giraud-Delville, Angéline Favre-Inhofer, Gilles Charpigny, Olivier Sandra, Nathalie Cornet, Jean-Marc Ayoubi, Romain Bosc, Aurélie Revaux, Marie Carbonnel, Pascale Chavatte-Palmer, Raphaël Coscas, Christophe Richard |
|---|---|
| Přispěvatelé: | Biologie de la Reproduction, Environnement, Epigénétique & Développement (BREED), École nationale vétérinaire - Alfort (ENVA)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Hôpital Foch [Suresnes], Service de chirurgie plastique et reconstructive [Mondor], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Hôpital Ambroise Paré [AP-HP], Centre de recherche en épidémiologie et santé des populations (CESP), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Paul Brousse-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay, The authors received funding (financial and material support) from the Foch Foundation. Angéline Favre-Inhofer and Nathalie Cornet were paid a stipend by the Foch Foundation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript., Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-École nationale vétérinaire d'Alfort (ENVA)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Chavatte-Palmer, Pascale |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
[SDV.MHEP.CHI] Life Sciences [q-bio]/Human health and pathology/Surgery
Physiology Uterus 030230 surgery Endometrium Vascular Medicine 0302 clinical medicine Ischemia Medicine and Health Sciences Immune Response 030219 obstetrics & reproductive medicine Multidisciplinary Anastomosis Surgical Arteries Dissection medicine.anatomical_structure Reperfusion Injury Gestation Medicine Female Anatomy Research Article medicine.medical_specialty Histology Science Immunology Urology [SDV.MHEP.CHI]Life Sciences [q-bio]/Human health and pathology/Surgery Anastomosis [SDV.MHEP.GEO]Life Sciences [q-bio]/Human health and pathology/Gynecology and obstetrics [SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction Veins 03 medical and health sciences Signs and Symptoms Uterus transplantation medicine Animals Vein Inflammation Sheep business.industry Reproductive System Biology and Life Sciences medicine.disease [SDV.MHEP.GEO] Life Sciences [q-bio]/Human health and pathology/Gynecology and obstetrics Reperfusion Cardiovascular Anatomy Blood Vessels Clinical Medicine business Physiological Processes Transcriptome |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2021, 16 (5), pp.e0251474. ⟨10.1371/journal.pone.0251474⟩ PLoS ONE, Vol 16, Iss 5, p e0251474 (2021) PLoS ONE, 2021, 16 (5), pp.e0251474. ⟨10.1371/journal.pone.0251474⟩ |
| ISSN: | 1932-6203 |
| Popis: | The dissection of the veins is the trickiest step of Uterine transplantation (UTx). Performing the anastomosis of a single uterine vein could bring a therapeutic benefit and simplification of surgery and serve for managing unilateral venous thromboses. The objectives of this project were to evaluate the expression of early markers of ischemia-reperfusion and to compare findings following one or two vein anastomoses. Orthotopic uterine auto-transplantations were performed on an ovine model with anastomosis of either two (group 1) or one utero-ovarian veins (group 2). Blood gases, histology and ischemia- reperfusion markers transcripts (PTGS2, IL6, IL8, SOD2, C3, BAX/BCL2 and TLR4) were analyzed as well as PTGS2 protein expression using Western Blot and fluorescence immunolocalization on endometrial biopsies after 3h of reperfusion. Ten ewes were included in the experimentation, 4 were in group1, 3 in group 2, the others being sham operated controls. No significant differences were observed between the two phenotypes. Based on these results, the anastomosis of one single uterine vein appears to be an approach consistent with short-term graft survival. Further experiments will be needed to confirm the reliability of this approach, especially the long-term follow-up of the uterine graft including its ability to support gestation to term. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|