Endothelin-1 and angiotensin II modulate rate and contraction amplitude in a subpopulation of mouse embryonic stem cell-derived cardiomyocyte-containing bodies
| Autor: | Colin W. Pouton, Ebba Louise Lagerqvist, John M. Haynes, Benjamin Arthur Llewellyn Finnin |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
medicine.medical_specialty
congenital hereditary and neonatal diseases and abnormalities Carbachol medicine.drug_class Video microscopy Biology Cell Line Mice Internal medicine Muscarinic acetylcholine receptor medicine Animals Myocytes Cardiac Receptor Embryonic Stem Cells Medicine(all) Endothelin-1 Angiotensin II Cell Differentiation General Medicine Cell Biology Receptor antagonist Endothelin 1 Myocardial Contraction Losartan Endocrinology cardiovascular system medicine.drug Developmental Biology |
| Zdroj: | Stem Cell Research. (1):23-33 |
| ISSN: | 1873-5061 |
| DOI: | 10.1016/j.scr.2010.09.001 |
| Popis: | Embryonic stem cell-derived cardiomyocytes (ESC-CMs) have applications in understanding cardiac disease pathophysiology, pharmacology, and toxicology. Comprehensive characterization of their basic physiological and pharmacological properties is critical in determining the suitability of ESC-CMs as models of cardiac activity. In this study we use video microscopy and quantitative PCR to investigate the responses of mouse ESC-CMs to adrenoceptor, muscarinic, angiotensin II (Ang II), and endothelin-1 (ET-1) receptor activation. Isoprenaline (10 nM–10 μM) increased beating rate and contraction amplitude in all beating bodies (BBs), whereas carbachol (up to 1 μM) and the I f channel blocker ZD-7288 (10 μM) decreased contraction frequency. ET-1 (0.01–100 nM) reduced contraction amplitude in all BBs and increased contraction frequency in 50% of BBs; these effects were blocked by the ET A receptor antagonist BQ123 (250 nM). Ang II (0.01 nM–1 μM) increased both contraction amplitude (all BBs) and frequency (in 50% of BBs), effects blocked, respectively, by losartan (100 nM) and PD123,319 (200 nM). These results indicate the presence of functional ET A and both AT 1 and AT 2 receptors in murine ESC-CMs, but their expression and or activity appears to be evident only in a limited set of BBs. |
| Databáze: | OpenAIRE |
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