Recombinant influenza A viruses harboring optimized dicistronic NA segment with an extended native 5′ terminal sequence: Induction of heterospecific B and T cell responses in mice
| Autor: | Nicolas Escriou, Nadia Naffakh, Sylvie van der Werf, Sylvie Gerbaud, Alexandre Vieira Machado |
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| Rok vydání: | 2006 |
| Předmět: |
Chloramphenicol O-Acetyltransferase
Male T-Lymphocytes viruses Genetic Vectors Heterologous Neuraminidase Live vaccine Subgenomic RNA Mengo virus medicine.disease_cause Virus Replication Viral vector law.invention Cell Line Mice Open Reading Frames Viral Proteins Dogs Influenza A Virus H1N1 Subtype law Virology Genomic Segment Dicistronic Chloramphenicol acetyltransferase Influenza A virus medicine Animals Gene Administration Intranasal Subgenomic mRNA B-Lymphocytes biology Viral Vaccines Molecular biology Recombinant Proteins Mice Inbred C57BL Reverse genetics biology.protein Recombinant DNA RNA Viral 5' Untranslated Regions |
| Zdroj: | Virology. 345(1):73-87 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/j.virol.2005.09.050 |
| Popis: | We generated novel recombinant influenza A viruses (vNA38) harboring dicistronic NA segments with an extended native 5′ terminal sequence of 70 nucleotides comprised of the last 42 nucleotides of the NA ORF and the 5′ noncoding region (5′ NCR). vNA38 viruses replicated stably and more efficiently than vNA35 viruses with a dicistronic NA segment comprised of the native 5′ NCR only, that we described previously (Vieira Machado, A., Naffakh, N., van der Werf, S., Escriou, N., 2003. Expression of a foreign gene by stable recombinant influenza viruses harboring a dicistronic genomic segment with an internal promoter. Virology 313, 235–249). In addition, vNA38 viruses drove the expression of higher levels of encoded heterologous proteins than corresponding vNA35 viruses, both in cell culture and in the pulmonary tissue of infected mice. These data demonstrate that a sequence overlapping 5′ coding and noncoding regions of the NA segment determines efficient replication and/or propagation of the vRNA. Intranasal immunization of mice with live vNA38 viruses induced B and T cell responses specific for the heterologous protein expressed, establishing the usefulness of such recombinant influenza viruses with a dicistronic segment for the development of live bivalent vaccines. |
| Databáze: | OpenAIRE |
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