Absence of mouse mammary tumor virus proviral amplification in chemically induced lymphomas of RF/J mice
| Autor: | Robert Kraft, Sharon A. Okenquist, Frank Lilly, S. T. Ishizaka, Jack Lenz, Geoffrey J. Childs |
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| Jazyk: | angličtina |
| Rok vydání: | 1989 |
| Předmět: |
Genes
Viral Lymphoma viruses Immunology Receptors Antigen T-Cell Mice Inbred Strains Gene Rearrangement T-Lymphocyte Microbiology Virus chemistry.chemical_compound Tissue culture Mice Virology Gene duplication medicine Animals biology Mouse mammary tumor virus Gene Amplification Nucleic Acid Hybridization DNA DNA Neoplasm Provirus medicine.disease biology.organism_classification chemistry Liver Mammary Tumor Virus Mouse Insect Science Methylcholanthrene Cancer research Oncovirus Research Article |
| Popis: | RF/J mice are susceptible to the induction of thymic lymphomas by the carcinogens 3-methylcholanthrene and N-methyl-N-nitrosourea. Given the association of mouse mammary tumor virus (MMTV) with certain thymomas, we examined genomic DNA from chemically induced lymphomas of RF/J mice for new MMTV proviruses. Of 13 tissue culture lines derived from 3-methylcholanthrene-induced tumors, 5 had acquired new proviruses. MMTV amplification coincided with the appearance of viral mRNAs and proteins. However, no primary tumors or animal-passaged tumors contained new proviruses. These observations indicate that MMTV does not have a role in the tumor induction process, although it may become activated and amplified in tissue culture lines derived from tumors. |
| Databáze: | OpenAIRE |
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