Use of ebselen as a neuroprotective agent in rat spinal cord subjected to traumatic injury
| Autor: | Edyta Olakowska, Izabella Woszczycka-Korczyńska, Władysław P. Węglarz, Wiesław Marcol, Daria Gendosz de Carrillo, Joanna Lewin-Kowalik, Wojciech Slusarczyk, Magdalena Larysz-Brysz |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
antioxidant functional recovery Scars Neuroprotection lcsh:RC346-429 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Developmental Neuroscience medicine ebselen neuroprotection neuroregeneration spinal cord injury spinal cord repair Spinal cord injury lcsh:Neurology. Diseases of the nervous system medicine.diagnostic_test Ebselen business.industry Magnetic resonance imaging Spinal cord medicine.disease Neuroregeneration 030104 developmental biology medicine.anatomical_structure chemistry Anesthesia medicine.symptom business 030217 neurology & neurosurgery Motor cortex Research Article |
| Zdroj: | Neural Regeneration Research, Vol 14, Iss 7, Pp 1255-1261 (2019) Neural Regeneration Research |
| ISSN: | 1673-5374 |
| Popis: | Spinal cord injury (SCI) causes disturbances of motor skills. Free radicals have been shown to be essential for the development of spinal cord trauma. Despite some progress, until now no effective pharmacological therapies against SCI have been verified. The purpose of our experiment was to investigate the neuroprotective effects of ebselen on experimental SCI. Twenty-two rats subjected to SCI were randomly subjected to SCI with no further treatment (n = 10) or intragastric administration of ebselen (10 mg/kg) immediately and 24 hours after SCI. Behavioral changes were assessed using the Basso, Beattie, and Bresnahan locomotor scale and footprint test during 12 weeks after SCI. Histopathological and immunohistochemical analyses of spinal cords and brains were performed at 12 weeks after SCI. Magnetic resonance imaging analysis of spinal cords was also performed at 12 weeks after SCI. Rats treated with ebselen presented only limited neurobehavioral progress as well as reduced spinal cord injuries compared with the control group, namely length of lesions (cysts/scars) visualized histopathologically in the spinal cord sections was less but cavity area was very similar. The same pattern was found in T2-weighted magnetic resonance images (cavities) and diffusion-weighted images (scars). The number of FluoroGold retrogradely labeled neurons in brain stem and motor cortex was several-fold higher in ebselen-treated rats than in the control group. The findings suggest that ebselen has only limited neuroprotective effects on injured spinal cord. All exprimental procedures were approved by the Local Animal Ethics Committee for Experiments on Animals in Katowice (Katowice, Poland) (approval No. 19/2009). |
| Databáze: | OpenAIRE |
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