In vitro assessment of the anti-sickling properties of Buchholzia coriacea and Mucuna pruriens seed extracts
| Autor: | PN Okafor, Eberechukwu Laura Ikechukwu, Simeon Ikechukwu Egba |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Osmosis Sodium Iron Hemoglobin Sickle Phytochemicals chemistry.chemical_element Anemia Sickle Cell Capparaceae Polymerization 03 medical and health sciences 0302 clinical medicine Antisickling Agents medicine Humans Buchholzia coriacea Amino Acids Sickled erythrocytes Minerals biology Traditional medicine Plant Extracts Erythrocyte fragility In vitro toxicology Cell Biology General Medicine biology.organism_classification Mucuna In vitro Red blood cell 030104 developmental biology medicine.anatomical_structure chemistry Solubility 030220 oncology & carcinogenesis Seeds Mucuna pruriens Developmental Biology Phytotherapy |
| Zdroj: | In vitro cellulardevelopmental biology. Animal. 56(9) |
| ISSN: | 1543-706X |
| Popis: | Sickle cell disease is a group of diseases inherited through the gene and it affects the haemoglobin in the red blood cell. This study investigated the methanol seed extract of Buchholzia coriacea for possible in vitro anti-sickling effects and also determined the effect of Mucuna pruriens seed extract on the solubility and oxygen-binding rate of sickle cell haemoglobin. Sickle cell blood was collected from sickle cell disease patients with subsequent addition of 2% sodium metabisulphite to cause more sickling. Varying concentrations of the seed extracts (50%, 25%, 12.5% and 6.25%) were added to the pre-treated blood for these in vitro assays. The results showed that the extract of Buchholzia coriacea significantly (P < 0.05) inhibited sickling at all concentrations with the highest percentage inhibition of 73.3 ± 5.8, reversed sickled erythrocytes at all concentrations with the highest percentage reversal of 83.3 ± 5.8 and significantly (P < 0.05) inhibited polymerisation at all concentrations used in comparison to the parallel control. The extract of Mucuna pruriens seed significantly (P < 0.05) increased the solubility of sickle haemoglobin at 50%, 25%, 12.5% and 6.25% concentrations, increased Fe2+/Fe3+ ratio from 1.7 (control) to 12.2 (50% concentration) and reduced osmotic fragility (at 12.5% and 6.25% concentrations) when compared with parallel control. The results indicate the feasibility of the seed extracts as promising agents in the management of sickle cell disease. |
| Databáze: | OpenAIRE |
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