Gene expression profiles of differentiated and undifferentiated adipose derived mesenchymal stem cells dynamically seeded onto a processed nerve allograft
| Autor: | Nadia Rbia, Roman Thaler, Alexander Y. Shin, Andre J. van Wijnen, Allen T. Bishop, Femke Mathot |
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| Přispěvatelé: | Plastic and Reconstructive Surgery and Hand Surgery |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Time Factors Cell Culture Techniques Neovascularization Physiologic Rats Sprague-Dawley Extracellular matrix 03 medical and health sciences 0302 clinical medicine Gene expression Genetics Glial cell line-derived neurotrophic factor Animals Transplantation Homologous Decellularization biology Nerve allograft Regeneration (biology) Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells General Medicine Allografts Sciatic Nerve Extracellular Matrix Nerve Regeneration Cell biology Women's cancers Radboud Institute for Health Sciences [Radboudumc 17] 030104 developmental biology Adipose Tissue Rats Inbred Lew 030220 oncology & carcinogenesis biology.protein Schwann Cells Transcriptome Neurotrophin |
| Zdroj: | Gene, 724 Gene, 724:Unsp 144151. Elsevier |
| ISSN: | 1879-0038 0378-1119 |
| Popis: | Contains fulltext : 229427.pdf (Publisher’s version ) (Closed access) BACKGROUND: Differentiation of mesenchymal stem cells (MSCs) into Schwann-like cells onto processed nerve allografts may support peripheral nerve repair. The purpose of this study was to understand the biological characteristics of undifferentiated and differentiated MSCs before and after seeding onto a processed nerve allograft by comparing gene expression profiles. METHODS: MSCs from Lewis rats were cultured in maintenance media or differentiated into Schwann-like cells. Both treatment groups were dynamically seeded onto decellularized nerve allografts derived from Sprague-Dawley rats. Gene expression was quantified by quantitative polymerase chain reaction (qPCR) analysis of representative biomarkers, including neurotrophic (GDNF, PTN, GAP43, PMP22), angiogenic (CD31, VEGF1), extracellular matrix (ECM) (COL1A1, COL3A1, FBLN1, LAMB2) or cell cycle (CAPS3, CCBN2) genes. Gene expression values were statistically evaluated using a 2-factor ANOVA with repeated measures. RESULTS: Baseline gene expression of undifferentiated and differentiated MSCs was significantly altered upon interaction with processed nerve allografts. Interaction between processed allografts and undifferentiated MSCs enhanced expression of neurotrophic (NGF, GDNF, PMP22), ECM (FBLN1, LAMB2) and regulatory cell cycle genes (CCNB2) during a 7-day time course. Interactions of differentiated MSCs with nerve allografts enhanced expression of neurotrophic (NGF, GDNF, GAP43), angiogenic (VEGF1), ECM (FBLN1) and regulatory cell cycle genes (CASP3, CCNB2) within one week. CONCLUSIONS: Dynamic seeding onto processed nerve allografts modulates temporal gene expression profiles of differentiated and undifferentiated MSCs. These changes in gene expressions may support the reparative functions of MSCs in supporting nerve regeneration in different stages of axonal growth. |
| Databáze: | OpenAIRE |
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