Estrogenic effects in flounder Platichthys flesus orally exposed to 4-tert-octylphenol
| Autor: | Louise Leonharder Madsen, Poul Bjerregaard, Bodil Korsgaard |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Muscle tissue
Male medicine.medical_specialty Health Toxicology and Mutagenesis Flounder Aquatic Science Pleuronectidae Vitellogenin Surface-Active Agents Vitellogenins Phenols Oral administration Internal medicine Testis medicine Animals Estrogens Non-Steroidal biology Dose-Response Relationship Drug Estradiol Chemistry Muscles Organ Size biology.organism_classification Platichthys Diet medicine.anatomical_structure Endocrinology Logistic Models Endocrine disruptor Liver Toxicity biology.protein |
| Zdroj: | University of Southern Denmark |
| ISSN: | 0166-445X |
| Popis: | The estrogenic effect of dietary 4- tert -octylphenol in the flounder Platichthys flesus was investigated in the laboratory. In the first experiment male flounders were fed doses of 10, 50 and 100 mg 4- tert -octylphenol kg −1 BW or 0.05 mg 17β-estradiol kg −1 BW every second day for 10 days. Increases in the plasma content of vitellogenin were observed on day 6 by the first sampling in all treated groups. An effect of octylphenol on the size of the liver and testis was only seen in one of the exposed group (50 mg 4- tert -octylphenol kg −1 BW). An increase in the concentration of 4- tert -octylphenol was found in both liver and muscle tissues of all 4- tert -octylphenol treated fish. The concentration of 4- tert -octylphenol (ng g −1 WW) in liver tissue was four to five times greater than the concentration in muscle tissue. Only about 8% of the 4- tert -octylphenol administered to the group receiving 50 mg 4- tert -octylphenol kg −1 BW was retained in the liver and the muscles at the end of the experiment. In the second experiment male flounders were exposed to a low dose range of 4- tert -octylphenol (1 to 25 mg 4- tert -octylphenol kg −1 BW) in order to establish the dose-response relationship of 4- tert -octylphenol when applied by feeding to flounder. Vitellogenin induction (LOED) occurred at 10 and 5 mg 4- tert -octylphenol kg −1 BW after, respectively, 6 and 11 days of exposure. Based on a logistic regression analysis the effect dose (ED 50 ) could be determined as 8.2 and 5.6 mg 4- tert -octylphenol kg −1 BW on day 6 and 11, respectively. 4- tert -octylphenol accumulated in liver, muscle and testis tissue, and a significant accumulation in liver was found at 2.5 mg 4- tert -octylphenol kg −1 BW and in muscle and testis at 5 mg 4- tert -octylphenol kg −1 BW. Finally, a positive correlation between the plasma vitellogenin concentration and the liver tissue concentration of 4- tert -octylphenol was established as well as a positive correlation between the content of 4- tert -octylphenol in liver and muscle/testis tissue. |
| Databáze: | OpenAIRE |
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