BK Channel Deficiency in Osteoblasts Reduces Bone Formation via the Wnt/β-Catenin Pathway
| Autor: | Jiahong Yang, Hong Xin, Qianhong Yang, Lan Jiang, Xuemei Zhang, Jianjun Gao, Jiaqi He |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
BK channel
Kcnma1 Western blot Osteogenesis AXIN1 medicine Animals Bone formation Large-Conductance Calcium-Activated Potassium Channels Molecular Biology Wnt Signaling Pathway beta Catenin Mice Knockout Osteoblasts medicine.diagnostic_test biology Chemistry Wnt signaling pathway Osteoblast Cell Differentiation Cell Biology General Medicine β-catenin Cell biology medicine.anatomical_structure bone development Catenin biology.protein Female Signal transduction Gene Deletion Research Article |
| Zdroj: | Molecules and Cells |
| ISSN: | 0219-1032 |
| Popis: | Global knockout of the BK channel has been proven to affect bone formation; however, whether it directly affects osteoblast differentiation and the mechanism are elusive. In the current study, we further investigated the role of BK channels in bone development and explored whether BK channels impacted the differentiation and proliferation of osteoblasts via the canonical Wnt signaling pathway. Our findings demonstrated that knockout of Kcnma1 disrupted the osteogenesis of osteoblasts and inhibited the stabilization of β-catenin. Western blot analysis showed that the protein levels of Axin1 and USP7 increased when Kcnma1 was deficient. Together, this study confirmed that BK ablation decreased bone mass via the Wnt/β-catenin signaling pathway. Our findings also showed that USP7 might have the ability to stabilize the activity of Axin1, which would increase the degradation of β-catenin in osteoblasts. |
| Databáze: | OpenAIRE |
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