Glutathione-Garlic Sulfur Conjugates: Slow Hydrogen Sulfide Releasing Agents for Therapeutic Applications

Autor: Maurizio Paci, Vilma Toska Papajani, Sonia Melino, Ashif I. Bhuiyan
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Antioxidant
medicine.medical_treatment
Hydrogen sulfide
Sulfur metabolism
hydrogen sulfide
Pharmaceutical Science
Analytical Chemistry
chemistry.chemical_compound
garlic
Thioredoxins
Drug Discovery
garlic hydrogen sulfide allyl sulfur compounds sulfurtransferase tumor cells
Chromatography
High Pressure Liquid
Thiosulfate
Chromatography
Reverse-Phase
food and beverages
Glutathione
sulfur conjugates
Cold Temperature
Biochemistry
Chemistry (miscellaneous)
Reducing Agents
Molecular Medicine
Organosulfur compounds
tumor cells
Allyl sulfur compounds
Extraction
Garlic
Sulfur conjugates
Sulfurtransferase
Tumor cells
chemistry.chemical_element
Lymphoma
T-Cell
allyl sulfur compounds
Article
lcsh:QD241-441
lcsh:Organic chemistry
Cell Line
Tumor
medicine
Humans
Settore BIO/10
Physical and Theoretical Chemistry
Cell Proliferation
Sulfur Compounds
Plant Extracts
Organic Chemistry
Water
Sulfur
Thiosulfate Sulfurtransferase
Molecular Weight
chemistry
Microscopy
Fluorescence
Solubility
Biocatalysis
extraction
sulfurtransferase
Zdroj: Molecules
Volume 20
Issue 1
Pages 1731-1750
Molecules, Vol 20, Iss 1, Pp 1731-1750 (2015)
ISSN: 1420-3049
DOI: 10.3390/molecules20011731
Popis: Natural organosulfur compounds (OSCs) from Allium sativum L. display antioxidant and chemo-sensitization properties, including the in vitro inhibition of tumor cell proliferation through the induction of apoptosis. Garlic water- and oil-soluble allyl sulfur compounds show distinct properties and the capability to inhibit the proliferation of tumor cells. In the present study, we optimized a new protocol for the extraction of water-soluble compounds from garlic at low temperatures and the production of glutathionyl-OSC conjugates during the extraction. Spontaneously, Cys/GSH-mixed-disulfide conjugates are produced by in vivo metabolism of OSCs and represent active molecules able to affect cellular metabolism. Water-soluble extracts, with (GSGaWS) or without (GaWS) glutathione conjugates, were here produced and tested for their ability to release hydrogen sulfide (H2S), also in the presence of reductants and of thiosulfate:cyanide sulfurtransferase (TST) enzyme. Thus, the TST catalysis of the H2S-release from garlic OSCs and their conjugates has been investigated by molecular in vitro experiments. The antiproliferative properties of these extracts on the human T-cell lymphoma cell line, HuT 78, were observed and related to histone hyperacetylation and downregulation of GAPDH expression. Altogether, the results presented here pave the way for the production of a GSGaWS as new, slowly-releasing hydrogen sulfide extract for potential therapeutic applications.
Databáze: OpenAIRE
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