The Functional Human C-Terminome
| Autor: | Sanguthevar Rajasekaran, Oniel Toledo, Steven B. Brooks, Michael W. Hedden, Vishal Thapar, Sean R. Williams, Justin Limtong, Roxanne P. David, Surbhi Sharma, Jacklyn M. Newsome, Martin R. Schiller, Nemanja Novakovic, Kenneth F. Lyon |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Proteomics Proteomes lcsh:Medicine Biochemistry Database and Informatics Methods Binding Analysis Protein sequencing Human proteome project lcsh:Science Databases Protein Peptide sequence Genetics Mammals Multidisciplinary Proteomic Databases Genomics Genomic Databases Proteome Vertebrates Sequence Analysis Cell Binding Assay Research Article Protein domain Molecular Sequence Data Sequence Databases Computational biology Biology Research and Analysis Methods Rodents 03 medical and health sciences Protein Domains Sequence Motif Analysis Consensus sequence Animals Humans Amino Acid Sequence Molecular Biology Techniques Sequencing Techniques Gene Molecular Biology Chemical Characterization lcsh:R Organisms Biology and Life Sciences Proteins Computational Biology Genome Analysis 030104 developmental biology Biological Databases Amniotes Human genome lcsh:Q |
| Zdroj: | PLoS ONE PLoS ONE, Vol 11, Iss 4, p e0152731 (2016) |
| ISSN: | 1932-6203 |
| Popis: | All translated proteins end with a carboxylic acid commonly called the C-terminus. Many short functional sequences (minimotifs) are located on or immediately proximal to the C-terminus. However, information about the function of protein C-termini has not been consolidated into a single source. Here, we built a new "C-terminome" database and web system focused on human proteins. Approximately 3,600 C-termini in the human proteome have a minimotif with an established molecular function. To help evaluate the function of the remaining C-termini in the human proteome, we inferred minimotifs identified by experimentation in rodent cells, predicted minimotifs based upon consensus sequence matches, and predicted novel highly repetitive sequences in C-termini. Predictions can be ranked by enrichment scores or Gene Evolutionary Rate Profiling (GERP) scores, a measurement of evolutionary constraint. By searching for new anchored sequences on the last 10 amino acids of proteins in the human proteome with lengths between 3-10 residues and up to 5 degenerate positions in the consensus sequences, we have identified new consensus sequences that predict instances in the majority of human genes. All of this information is consolidated into a database that can be accessed through a C-terminome web system with search and browse functions for minimotifs and human proteins. A known consensus sequence-based predicted function is assigned to nearly half the proteins in the human proteome. Weblink: http://cterminome.bio-toolkit.com. |
| Databáze: | OpenAIRE |
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