Does the solid-state structure of endothelin-1 provide insights concerning the solution-state conformational equilibrium?
| Autor: | Thomas M. Marschner, Niels H. Andersen, Gregory M. Lee, Chinpan Chen |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
medicine.hormone
Magnetic Resonance Spectroscopy Protein Conformation Molecular Sequence Data Biophysics Detection of helicity Conformation X-ray vs. NMR Crystallography X-Ray Biochemistry Endothelin Protein Structure Secondary Endothelins chemistry.chemical_compound Protein structure Structural Biology Genetics medicine Molecule Amino Acid Sequence Acetonitrile Molecular Biology Molecular Structure Chemistry Chemical shift Intermolecular force Cell Biology Nuclear magnetic resonance spectroscopy Solutions Crystallography Helix |
| Zdroj: | FEBS letters. 355(2) |
| ISSN: | 0014-5793 |
| Popis: | Additional NMR data (local NOE ratios and chemical shifts) for endothelin-1 supporting the existence of a relatively regular helix initiated abruptly at Lys9 (with Asp8 as an N-cap) and extending in all cases to Cys15 (and in a frayed form to Asp18 in some analogs) is presented. The recent solid-state structure [Janes et al. (1994), Nature Struct. Biol. 1, 311–319], in contrast, places the helix in the extreme C-terminal section of the structure and the Lys9—Tyr13 segment is not helical. The X-ray structure does not predict the NOEs or chemical shifts observed for endothelins in aqueous media containing polar organic co-solvents. An analysis of the chemical shift data for reporter groups indicates that the helical conformational preference of endothelins is not significantly altered by the addition of acetonitrile, acetic acid, or ethylene glycol. The validity of the analytic strategy is supported by results for both more rigid and less helical analogs. We conclude that the structure observed in crystals obtained from purely aqueous media is influenced by intermolecular interactions in the solid state and is not a significant contributor to the conformational equilibrium observed for monomeric ET-1. |
| Databáze: | OpenAIRE |
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