Electroacupuncture Delays Cartilage Degeneration by Modulating Nuclear Factor-κB Signaling Pathway
| Autor: | Yunmei Huang, Mingxia Wu, Cai-bin Pan, Wei Lin, Wenlie Chen, Guangwen Wu, Jun Chen, Xianxiang Liu, Shi-mao Zhang |
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| Rok vydání: | 2018 |
| Předmět: |
Cartilage
Articular Male medicine.medical_specialty Electroacupuncture medicine.medical_treatment Interleukin-1beta 0211 other engineering and technologies 02 engineering and technology IκB kinase Osteoarthritis 030226 pharmacology & pharmacy 03 medical and health sciences 0302 clinical medicine Chondrocytes Western blot NF-KappaB Inhibitor alpha Internal medicine 021105 building & construction Synovial Fluid Medicine Synovial fluid Animals Pharmacology (medical) medicine.diagnostic_test business.industry Interleukin-6 Tumor Necrosis Factor-alpha Cartilage NF-kappa B Transcription Factor RelA General Medicine medicine.disease I-kappa B Kinase medicine.anatomical_structure Endocrinology Complementary and alternative medicine Tumor necrosis factor alpha Matrix Metalloproteinase 3 Rabbits Signal transduction business Signal Transduction |
| Zdroj: | Chinese journal of integrative medicine. 25(9) |
| ISSN: | 1672-0415 |
| Popis: | To illustrate the molecular mechanisms underlying the therapeutic effects of electroacupuncture (EA) on knee osteoarthritis (OA). Twenty-seven six-month-old New Zealand white rabbits were allocated into three groups in accordance with a random number table: normal group (no surgery-induced OA; without treatment), model group (surgery-induced OA; without treatment) and EA group [surgery-induced OA; received treatment with EA at acupoints Dubi (ST 35) and Neixiyan (EX-LE 5), 30 min twice a day]. After eight consecutive weeks of treatment, the histopathological alterations in cartilage were observed using optical microscopy and transmission electron microscopy, cartilage degeneration was evaluated by modified Mankin’s score principles, the synovial fluid concentration of interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and matrix metalloproteinase-3 (MMP-3) were evaluated by enzyme-linked immunosorbent assay, and the protein expression levels of IL-1β, IL-6, TNF-α, MMP-3, IκB kinase-β (IKK-β), nuclear factor of α light polypeptide gene enhancer in B-cells inhibitor α (IκB-α) and nuclear factor-κB (NF-κB) p65 were quantified by Western blot analysis. EA treatment significantly improved cartilage structure arrangement and reduced cellular degeneration. The IL-1β, IL-6, TNF-α and MMP-3 of synovial fluid in the EA-treated group were significantly decreased compared with the model group (all P |
| Databáze: | OpenAIRE |
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