The ventrolateral preoptic nucleus is not required for isoflurane general anesthesia
| Autor: | Shigefumi Yokota, Matthias Eikermann, Nancy L. Chamberlin, Clifford B. Saper, Ramalingam Vetrivelan, Ulrike Hoffmann, Mark Henry, Martina Grosse-Sundrup |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Male
medicine.medical_specialty Anesthetics General Medizin Polysomnography Anesthesia General Article Rats Sprague-Dawley Internal medicine medicine Animals Molecular Biology Neurons Isoflurane medicine.diagnostic_test business.industry General Neuroscience Preoptic Area Rats Sleep deprivation Burst suppression medicine.anatomical_structure Endocrinology Hypothalamus Anesthesia Anesthetic Neurology (clinical) Neuron Righting reflex medicine.symptom Sleep business Developmental Biology medicine.drug |
| Popis: | Neurons of the ventrolateral preoptic nucleus (VLPO) promote sleep and VLPO loss produces insomnia. Previous studies show that general anesthetics including isoflurane activate VLPO neurons, and may contribute to their sedative effects. However, it is not clear to what extent the activation of VLPO neurons contributes to general anesthesia. We tested whether destruction of the VLPO neurons would affect the onset, depth, or recovery from isoflurane's general anesthetic effects. The VLPO was ablated in 25 rats by bilateral local injection of orexin-saporin, and polysomnography was performed to measure baseline sleep loss and responses to isoflurane anesthesia at 1% and 2%. Eight rats received sham (saline) injections. We measured isoflurane effects on time to loss of righting reflex, onset of continuous slow wave activity, and burst suppression; burst-suppression ratio; and time to recovery of righting reflex and desynchronized EEG. VLPO neuron cell loss was quantified by post hoc histology. Loss of VLPO neurons as well as lesion size were associated with cumulative sleep loss (r = 0.77 and r = 0.62, respectively), and cumulative sleep loss was the strongest predictor of high sensitivity to anesthesia, expressed as decreased time to loss of righting reflex (− 0.59), increased burst-suppression ratio (r = 0.52) , and increased emergence time (r = 0.54); an interaction-effect of isoflurane dose was observed (burst-suppression ratio: p |
| Databáze: | OpenAIRE |
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