Differential Contribution of Plant-Beneficial Functions from Pseudomonas kilonensis F113 to Root System Architecture Alterations in Arabidopsis thaliana and Zea mays
| Autor: | Daniel Muller, Jordan Vacheron, Guilhem Desbrosses, Rosa Padilla, Claire Prigent-Combaret, Vincent Walker, Sébastien Renoud |
|---|---|
| Přispěvatelé: | Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Ecologie Microbienne (LEM ), prigent-combaret, claire, Ecologie microbienne ( EM ), Centre National de la Recherche Scientifique ( CNRS ) -Ecole Nationale Vétérinaire de Lyon ( ENVL ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique ( INRA ) -VetAgro Sup ( VAS ), Biochimie et Physiologie Moléculaire des Plantes ( BPMP ), Centre international d'études supérieures en sciences agronomiques ( Montpellier SupAgro ) -Institut national de la recherche agronomique [Montpellier] ( INRA Montpellier ) -Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS ) -Institut national d’études supérieures agronomiques de Montpellier ( Montpellier SupAgro ), Ecologie Microbienne ( LEM ), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro) |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
[ SDV.BV ] Life Sciences [q-bio]/Vegetal Biology co-occurring plant-beneficial functions maize Arabidopsis functional interactions fluorescent Pseudomonas maïs Physiology 030106 microbiology Mutant Pseudomonas fluorescens Rhizobacteria Plant Roots Zea mays 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins Biosynthesis Glucose dehydrogenase Pseudomonas [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Arabidopsis thaliana interaction fonctionnelle Symbiosis relation plante-bactérie 2. Zero hunger Vegetal Biology biology food and beverages Gene Expression Regulation Bacterial General Medicine biology.organism_classification Nitrite reductase Biochemistry chemistry pseudomonas fluorescens Agronomy and Crop Science Gene Deletion Biologie végétale |
| Zdroj: | Molecular Plant-Microbe Interactions Molecular Plant-Microbe Interactions, American Phytopathological Society, 2017, ⟨10.1094/MPMI-07-17-0185-R⟩ Molecular Plant-Microbe Interactions 2 (31), 212-223. (2018) Molecular Plant-Microbe Interactions, American Phytopathological Society, 2017, 〈10.1094/MPMI-07-17-0185-R〉 Molecular Plant-Microbe Interactions, American Phytopathological Society, 2017, 31 (2), ⟨10.1094/MPMI-07-17-0185-R⟩ |
| ISSN: | 1943-7706 0894-0282 |
| DOI: | 10.1094/mpmi-07-17-0185-r |
| Popis: | Fluorescent pseudomonads are playing key roles in plant-bacteria symbiotic interactions due to the multiple plant-beneficial functions (PBFs) they are harboring. The relative contributions of PBFs to plant-stimulatory effects of the well-known plant growth-promoting rhizobacteria Pseudomonas kilonensis F113 (formerly P. fluorescens F113) were investigated using a genetic approach. To this end, several deletion mutants were constructed, simple mutants ΔphlD (impaired in the biosynthesis of 2,4-diacetylphloroglucinol [DAPG]), ΔacdS (deficient in 1-aminocyclopropane-1-carboxylate deaminase activity), Δgcd (glucose dehydrogenase deficient, impaired in phosphate solubilization), and ΔnirS (nitrite reductase deficient), and a quadruple mutant (deficient in the four PBFs mentioned above). Every PBF activity was quantified in the wild-type strain and the five deletion mutants. This approach revealed few functional interactions between PBFs in vitro. In particular, biosynthesis of glucose dehydrogenase severely reduced the production of DAPG. Contrariwise, the DAPG production impacted positively, but to a lesser extent, phosphate solubilization. Inoculation of the F113 wild-type strain on Arabidopsis thaliana Col-0 and maize seedlings modified the root architecture of both plants. Mutant strain inoculations revealed that the relative contribution of each PBF differed according to the measured plant traits and that F113 plant-stimulatory effects did not correspond to the sum of each PBF relative contribution. Indeed, two PBF genes (ΔacdS and ΔnirS) had a significant impact on root-system architecture from both model plants, in in vitro and in vivo conditions. The current work underscored that few F113 PBFs seem to interact between each other in the free-living bacterial cells, whereas they control in concert Arabidopsis thaliana and maize growth and development. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|