Imaging and Targeting of the α(2–6) and α(2–3) Linked Sialic Acid Quantum Dots in Zebrafish and Mouse Models
| Autor: | Rohan Yadav, Harikrishna Bavireddi, Raghavendra Kikkeri, Raghavendra Vasudeva Murthy, Balamurugan Subramani, Suraj Toraskar, Preeti Madhukar Chaudhary, Sivakoti Sangabathuni |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Biodistribution Materials science Danio 02 engineering and technology Mice 03 medical and health sciences chemistry.chemical_compound In vivo Quantum Dots Animals Tissue Distribution General Materials Science Zebrafish biology technology industry and agriculture equipment and supplies 021001 nanoscience & nanotechnology biology.organism_classification N-Acetylneuraminic Acid Sialic acid Mice Inbred C57BL 030104 developmental biology chemistry Drug delivery Biophysics Nanocarriers 0210 nano-technology N-Acetylneuraminic acid |
| Zdroj: | ACS Applied Materials & Interfaces. 10:28322-28330 |
| ISSN: | 1944-8252 1944-8244 |
| DOI: | 10.1021/acsami.8b07668 |
| Popis: | Sialic acid-conjugated nanocarriers have emerged as attractive biomarkers with promising biomedical applications. The translation of these nanocarriers into clinical applications requires in-depth assessment in animal models. However, due to the complexity, ethical concerns, and cost of the high-order animal system, there is an immediate need of information-rich simple animal models to decipher the biological significance. Herein, we performed in vivo head-to-head comparison of Neu5Acα(2-6) and α(2-3)Gal conjugated quantum dots (QDs) toxicity, biodistribution, and sequestration in wild-type zebrafish ( Danio rerio) and mouse model (C57BL). The fluorescent properties and cadmium composition of quantum dots were used to map the blood clearance, biodistribution, and sequestration of the sialylated QDs in major organs of both models. We observed that α(2-6) sialylated QDs preferentially have prolonged circulating half-life and broader biodistribution in both models. On the contrary, α(2-3) sialic acid and galactose-conjugated QDs have shortened blood circulation time and are sequestered in the liver, and cleared after several hours in both models. These results demonstrate the applicability of the zebrafish and sialylated QDs to target specific organs, as well as drug delivery and biomedical diagnostics. |
| Databáze: | OpenAIRE |
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