A capillary electrophoresis based approach for the identification of anti-drug antibodies against camelid VHH biologics (Nanobodies®)
| Autor: | Derek Wiswell, Enrique Escandón, Alissa A. Chackerian, Shuli Zhang, Edward P. Bowman, Divas Neupane, Anandi Sawant, Douglas Linn, Minchao Chen |
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| Rok vydání: | 2020 |
| Předmět: |
Drug
media_common.quotation_subject Computational biology 030204 cardiovascular system & hematology Toxicology 030226 pharmacology & pharmacy Epitope 03 medical and health sciences 0302 clinical medicine Capillary electrophoresis medicine Animals media_common Pharmacology Biological Products Mice Inbred BALB C Experimental drug medicine.diagnostic_test biology Chemistry Immunogenicity Antibodies Monoclonal Electrophoresis Capillary Serum samples Mice Inbred C57BL Immunoassay biology.protein Drug Monitoring Antibody |
| Zdroj: | Journal of Pharmacological and Toxicological Methods. 103:106872 |
| ISSN: | 1056-8719 |
| DOI: | 10.1016/j.vascn.2020.106872 |
| Popis: | Undesired immune responses against protein therapeutics may adversely affect the pharmacokinetics, efficacy, and safety of the product. The presence of anti-drug-antibodies (ADA) has been the key determinant of immunogenic responses. Here we describe the use of a capillary electrophoresis platform for the identification of ADAs against several experimental camelid VHH biologics (Nanobodies®). Hereafter, we refer to this assay as WESADA. We modified the Wes platform by ProteinSimple to screen serum samples for ADA against covalently linked multi-modular Nanobodies and compared it to standard ADA methodologies. We were able to identify ADA positive samples and determine which individual VHH module in a multivalent Nanobody construct stimulated the predominant ADA response. WESADA requires denaturation of the experimental immobilized drug, which could affect recognition of the immunogenic epitope and alter ADA signal. To address this issue, we demonstrated that signal can be immunodepleted by pre-incubation of serum samples with native Nanobody. This capillary electrophoresis based approach allows for rapid analysis without the need for individually tailored assay optimization or reagent labeling, while consuming small amounts of sample and drug. It also allows for the simultaneous ADA analysis of multiple targets of different molecular size in the same experimental sample. WESADA is not intended to replace traditional ADA assay formats, but it facilitates the expedient immunogenic assessment of a large number of experimental drug candidates in the early developmental space. |
| Databáze: | OpenAIRE |
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