Biophysical properties of Saccharomyces cerevisiae and their relationship with HOG pathway activation
| Autor: | Edda Klipp, Mattias Goksör, Stefan Hohmann, Emma Eriksson, Francesc Posas, Mathias Peter, Jörg Schaber, Serge Pelet, Miquel Àngel Adrover, Dagmara Medrala Klein, Elzbieta Petelenz-Kurdziel |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Glycerol
0106 biological sciences Volumetric elastic modulus Osmotic shock Turgor pressure Intracellular Space Biophysics Saccharomyces cerevisiae Biology Cell wall elasticity Models Biological Plasmolysis 010603 evolutionary biology 01 natural sciences Biophysical Phenomena Cell wall 03 medical and health sciences Cell Wall Osmotic Pressure Elastic Modulus Turgor High osmolarity glycerol (HOG) signaling Elasticity (economics) Elastic modulus 030304 developmental biology Original Paper 0303 health sciences Osmotic concentration Cell Membrane Reproducibility of Results General Medicine Yeast Biochemistry Thermodynamics Saccharomyces cerevisiae -- Metabolisme Model discrimination Extracellular Space Membranes cel·lulars Intracellular Signal Transduction |
| Zdroj: | Recercat. Dipósit de la Recerca de Catalunya instname European Biophysics Journal; Vol 39 European Biophysics Journal |
| ISSN: | 1432-1017 0175-7571 |
| DOI: | 10.1007/s00249-010-0612-0 |
| Popis: | Parameterized models of biophysical and mechanical cell properties are important for predictive mathematical modeling of cellular processes. The concepts of turgor, cell wall elasticity, osmotically active volume, and intracellular osmolarity have been investigated for decades, but a consistent rigorous parameterization of these concepts is lacking. Here, we subjected several data sets of minimum volume measurements in yeast obtained after hyper-osmotic shock to a thermodynamic modeling framework. We estimated parameters for several relevant biophysical cell properties and tested alternative hypotheses about these concepts using a model discrimination approach. In accordance with previous reports, we estimated an average initial turgor of 0.6 ± 0.2 MPa and found that turgor becomes negligible at a relative volume of 93.3 ± 6.3% corresponding to an osmotic shock of 0.4 ± 0.2 Osm/l. At high stress levels (4 Osm/l), plasmolysis may occur. We found that the volumetric elastic modulus, a measure of cell wall elasticity, is 14.3 ± 10.4 MPa. Our model discrimination analysis suggests that other thermodynamic quantities affecting the intracellular water potential, for example the matrix potential, can be neglected under physiological conditions. The parameterized turgor models showed that activation of the osmosensing high osmolarity glycerol (HOG) signaling pathway correlates with turgor loss in a 1:1 relationship. This finding suggests that mechanical properties of the membrane trigger HOG pathway activation, which can be represented and quantitatively modeled by turgor. This work was supported via several projects funded by the European Commission: QUASI (Contract No. 503230 to SH, EK, FP and MP), CELLCOMPUT (Contract No. 043310 to SH, EK and FP), UNICELLSYS (Contract No. 201142 to SH, EK, FP, MP and MG), SYSTEMSBIOLOGY (Contract No. 514169 to SH and EK), and AMPKIN (Contract No. 518181 to SH and MG). In addition work was funded by grants from the Swedish Foundation for Strategic Research SSF (Bio-X to MG), the Swedish Research Council (project grants to SH and MG), the Carl Trygger Foundation (to MG), the Science Faculty, University of Gothenburg (to SH and MG), and the Swiss systemsX.ch (to MP) |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|