Efficacy of two DNA fingerprinting methods for typing Acinetobacter baumannii isolates
| Autor: | Liliana S. Quelle, Mariana Catalano |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
DNA
Bacterial Microbiology (medical) Concordance Population Biology Polymerase Chain Reaction Sensitivity and Specificity Microbiology law.invention law Humans Typing education Polymerase chain reaction Palindromic sequence Genetics Cross Infection education.field_of_study Acinetobacter Reproducibility of Results DNA Patterns General Medicine biology.organism_classification DNA Fingerprinting Bacterial Typing Techniques Acinetobacter baumannii Infectious Diseases DNA profiling |
| Zdroj: | Diagnostic Microbiology and Infectious Disease. 39:215-223 |
| ISSN: | 0732-8893 |
| DOI: | 10.1016/s0732-8893(01)00238-3 |
| Popis: | Performance of macrorestriction and repetitive extragenic palindromic DNA sequence-based PCR (REP-PCR) to type Acinetobacter baumannii isolates was quantitatively estimated using a test population of 54 outbreak-related, 29 endemic infection-related and 17 epidemiologically-unrelated isolates. Reproducibility and stability for macrorestriction were 100%, and REP-PCR showed only slightly lower stability. Macrorestriction resolved 18 fingerprints and REP-PCR 10 DNA patterns, forming eight and seven clusters at 75% of similarity level, respectively. Intercluster band variation was7 bands for both methods. Although, all endemic isolates, except one, were concordantly grouped by both methods, macrorestriction distinguished a greater number of subtypes over one year study. For outbreaks, the epidemiologic concordance for both methods was 88%. The discriminatory index for macrorestriction and REP-PCR was 0.884 and 0.877, respectively. In conclusion, both methods showed similar efficacy as epidemiological markers, and by concordance, this study demonstrated that for REP-PCR typing, aor = 7 bands difference seemed an appropriate threshold to identify unrelated strains. |
| Databáze: | OpenAIRE |
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