Transmission-blocking antibodies recognize microfilarial chitinase in brugian lymphatic filariasis
| Autor: | Francine Perler, Randall F. Smith, William S. Lane, Juliet A. Fuhrman, Willy F. Piessens |
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| Jazyk: | angličtina |
| Rok vydání: | 1992 |
| Předmět: |
medicine.drug_class
Molecular Sequence Data Monoclonal antibody Microfilaria Polymerase Chain Reaction Brugia malayi Elephantiasis Filarial Antigen parasitic diseases medicine Brugia Parasite hosting Animals Amino Acid Sequence RNA Messenger Cloning Molecular Lymphatic filariasis Multidisciplinary biology Base Sequence Chitinases DNA biology.organism_classification medicine.disease Virology Peptide Fragments Oligodeoxyribonucleotides Antigens Helminth Chitinase biology.protein Antibody Sequence Alignment Research Article |
| Popis: | Brugia malayi is a parasitic nematode that causes lymphatic filariasis in humans. The monoclonal antibody MF1, which mediates clearance of peripheral microfilaremia in a gerbil infection model, recognizes two stage-specific proteins, p70 and p75, in B. malayi microfilariae. cDNA coding for the MF1 antigen was sequenced, and the predicted protein sequence shows significant similarities to chitinases from bacteria and yeast. When microfilarial extracts and purified preparations of the MF1 antigen were tested for chitinase activity, strong bands of chitin-degrading activity comigrated in SDS/PAGE with p70 and p75 and showed a reduction-dependent mobility shift characteristic of the MF1 antigen. Thus, the MF1 antigen is microfilarial chitinase, which may function to degrade chitin-containing structures in the microfilaria or in its mosquito vector during parasite development and transmission. |
| Databáze: | OpenAIRE |
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