Development and characterization of two new cell lines from common carp, Cyprinus carpio (Linn)
| Autor: | T. Rajaswaminathan, Gaurav Rathore, Mukunda Goswami, Wazir S Lakra |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
biology
mtDNA Growth factor medicine.medical_treatment General Medicine Anatomy cell line biology.organism_classification Molecular biology General Biochemistry Genetics and Molecular Biology In vitro Cyprinus Common carp lcsh:Biology (General) Cyprinus carpio Cell culture medicine biology.protein Cytochrome c oxidase Ploidy General Agricultural and Biological Sciences lcsh:QH301-705.5 Fetal bovine serum |
| Zdroj: | Biological Research, Volume: 43, Issue: 4, Pages: 385-392, Published: 2010 Scopus-Elsevier Biological Research v.43 n.4 2010 SciELO Chile CONICYT Chile instacron:CONICYT Biological Research, Vol 43, Iss 4, Pp 385-392 (2010) |
| Popis: | Two new cell lines (CCF and CCH) were established from fin and heart tissues of common carp, Cyprinus carpio. The cells were optimally maintained in Leibovitz-15 medium supplemented with 10% fetal bovine serum (FBS) and 10 ng/m1 of basic fibroblastic growth factor (bFGF). The effects of temperature, concentration of FBS and bFGF on the growth of CCF and CCH cells were examined. The temperature ranged from 24 to 32 °C for good growth of the cells. The growth rate of cells was higher in medium containing 10% FBS and the addition of bFGF to the medium significantly increased the growth rate. The CCF cells were found to be epithelial, while the CCH cells were fibroblastic in nature. The cytogenetic analysis of the cell lines revealed a diploid number of 100 chromosomes in C. carpio. The viability of CCF and CCH cell lines were 70 and 72%, respectively, after six months of storage in liquid nitrogen (-196 0 C). Molecular characterization of the cell lines using 16S rRNA and Cytochrome Oxidase Subunit I (COI) revealed the origin of the cell lines. These new cell lines will be useful for isolation of fish viruses and other in vitro biotechnological studies Key terms: Cyprinus carpio, cell line, mtDNA. |
| Databáze: | OpenAIRE |
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