Glial-cell-mediated re-induction of the blood-brain barrier phenotype in brain capillary endothelial cells: A differential gel electrophoresis study
| Autor: | Philippe Chafey, Cédric Broussard, Yannis Karamanos, Barbara Deracinois, Gwënaël Pottiez, Luc Camoin, Tom Teerlink, Pierre-Olivier Couraud, R. Cecchelli, Christophe Flahaut, Mariska Davids, Marie-Pierre Dehouck |
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| Přispěvatelé: | Laboratoire de Physiopathologie de la Barrière Hémato-Encéphalique (LBHE), Université d'Artois (UA), Clinical chemistry, ICaR - Circulation and metabolism |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
[SDV]Life Sciences [q-bio]
Immunoblotting asymmetric dimethylarginine Motility blood brain barrier Biology Proteomics Blood–brain barrier Arginine Biochemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine nitric oxide medicine Animals Electrophoresis Gel Two-Dimensional Molecular Biology DIGE 030304 developmental biology 0303 health sciences Reproducibility of Results Animal proteomics Phenotype In vitro endothelial cells Cell biology Culture Media Rats medicine.anatomical_structure chemistry Blood-Brain Barrier cardiovascular system Cattle Asymmetric dimethylarginine Neuroglia 030217 neurology & neurosurgery Intracellular Astrocyte |
| Zdroj: | Proteomics Proteomics, Wiley-VCH Verlag, 2013, 13 (7), pp.1185-1199. ⟨10.1002/pmic.201200166⟩ Journal of Proteomics, 13(7), 1185-1199. Elsevier Deracinois, B, Pottiez, G, Chafey, P, Teerlink, T, Camoin, L, Davids, M, Broussard, C, Couraud, P O, Dehouck, M P, Cecchelli, R, Karamanos, Y & Flahaut, C 2013, ' Glial-cell-mediated re-induction of the blood-brain barrier phenotype in brain capillary endothelial cells: A differential gel electrophoresis study ', Journal of Proteomics, vol. 13, no. 7, pp. 1185-1199 . https://doi.org/10.1002/pmic.201200166 PROTEOMICS; Vol 13 PROTEOMICS |
| ISSN: | 1615-9853 1615-9861 1874-3919 |
| Popis: | International audience; In the neurovascular unit, brain microvascular endothelial cells develop characteristic barrier features that control the molecular exchanges between the blood and the brain. These characteristics are partially or totally lost when the cells are isolated for use in in vitro blood-brain barrier (BBB) models. Hence, the re-induction of barrier properties is crucial for the relevance of BBB models. Although the role of astrocyte promiscuity is well established, the molecular mechanisms of re-induction remain largely unknown. Here, we used a DIGE-based proteomics approach to study endothelial cellular proteins showing significant quantitative variations after BBB re-induction. We confirm that quantitative changes mainly concern proteins involved in cell structure and motility. Furthermore, we describe the possible involvement of the asymmetric dimethylarginine pathway in the BBB phenotype re-induction process and we discuss asymmetric dimethylarginine's potential role in regulating endothelial function (in addition to its role as a by-product of protein modification). Our results also suggest that the intracellular redox potential is lower in the in vitro brain capillary endothelial cells displaying re-induced BBB functions than in cells with limited BBB functions. |
| Databáze: | OpenAIRE |
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