A Novel Technique for Redox Lipidomics Using Mass Spectrometry: Application on Vegetable Oils Used to Fry Potatoes
| Autor: | Shunji Kato, Traycie L. Williams, Yurika Otoki, Maria Fedorova, Elizabeth Z. Lin, Timothy J. Garrett, John A. Bowden, Krystal J. Godri Pollitt, Zhixu Ni, Juan J. Aristizabal-Henao, Meera Penumetcha, Kiyotaka Nakagawa, Jeremy P. Koelmel, Vasilis Vasiliou, Joy Guingab |
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| Rok vydání: | 2021 |
| Předmět: |
Novel technique
Deep frying 010402 general chemistry Mass spectrometry Tandem mass spectrometry 01 natural sciences Redox Tandem Mass Spectrometry Structural Biology Lipidomics medicine Plant Oils Cooking Food science Spectroscopy Solanum tuberosum chemistry.chemical_classification Chemistry 010401 analytical chemistry 0104 chemical sciences Castor oil Oxidation-Reduction Chromatography Liquid medicine.drug Polyunsaturated fatty acid |
| Zdroj: | Journal of the American Society for Mass Spectrometry. 32:1798-1809 |
| ISSN: | 1879-1123 1044-0305 |
| Popis: | Vegetables oils, rich in polyunsaturated fatty acids, are vulnerable to oxidation during manufacturing, processing, and food preparation. Currently, individual oxidation products are not well characterized, and hence, the health impacts of these unique lipid species remain unknown. Here, we introduce an extensive oxidized lipidomics in silico tandem mass spectrometry library and integrate these libraries within a user-friendly software covering a comprehensive redox lipidomics workflow. We apply this workflow to olive, soy, and walnut cooking oil; comparing unheated oil, oil after deep frying potatoes, and oil after oven frying potatoes. We annotated over a thousand oxidized triglycerides across 273 features (many coeluted). This software was validated against traditional chemical assays of oxidation, known oxidized lipids in castor oil, synthesized standards, and an alternate software LPPtiger. Development of these new software programs for redox lipidomics opens the door to characterize health implications of individual oxidation products. |
| Databáze: | OpenAIRE |
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