Substitution of egg yolk by a cyclodextrin-cholesterol complex allows a reduction of the glycerol concentration into the freezing medium of equine sperm
Autor: | Johann Detilleux, Didier Blommaert, Isabelle Donnay, Didier Serteyn, Thierry Franck, Jean-Philippe Lejeune |
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Rok vydání: | 2015 |
Předmět: |
Glycerol
Male food.ingredient Biology Semen analysis General Biochemistry Genetics and Molecular Biology Cryopreservation law.invention Andrology 03 medical and health sciences chemistry.chemical_compound Semen quality 0302 clinical medicine food Cryoprotective Agents law Semen Yolk Freezing medicine Animals Humans Food science Horses Sperm motility 030219 obstetrics & reproductive medicine medicine.diagnostic_test Extender beta-Cyclodextrins 0402 animal and dairy science 04 agricultural and veterinary sciences General Medicine 040201 dairy & animal science Sperm Egg Yolk Spermatozoa 2-Hydroxypropyl-beta-cyclodextrin Semen Analysis Cholesterol chemistry Sperm Motility General Agricultural and Biological Sciences Semen Preservation |
Zdroj: | Cryobiology. 72(1) |
ISSN: | 1090-2392 |
Popis: | The aim of this work was to completely replace the egg yolk a classical diluent for freezing equine semen by a cyclodextrin-cholesterol complex. At the same time, the reduction in the glycerol content used for cryopreservation and the incubation time between sperm and the freezing media were evaluated. Horse ejaculates were frozen with four different freezing extenders: a frozen reference medium (IF) containing egg yolk and 2.5% glycerol and media without egg yolk but supplemented with 1.5 mg 2-hydroxypropyl-beta-cyclodextrin cholesterol (HPβCD-C) complex and containing either 1% (G1), 2% (G2) or 3% glycerol (G3). Three incubation times (90, 120 and 180 min) at 4 °C between the fresh semen and the different media were tested before freezing. Viability and motility analyses were performed with computer assisted semen analysis (CASA). Results showed that the freezing media containing the HPβCD-C complex with 1%, 2% and 3% glycerol significantly improve the 3 in vitro parameters of post thawing semen quality (viability, progressive and total mobilities) compared to IF. The best improvement of the parameters was obtained with G1 medium and the longest contact time. The substitution of egg yolk by HPβCD-C complex allows the decrease of protein charge of the medium while favouring the cholesterol supply to membrane spermatozoa offering it a better resistance to osmotic imbalance and a better tolerance to the glycerol toxicity. Our results highlight that the egg yolk of an extender for the freezing of horse semen can be completely substituted by HPβCD-C complex. |
Databáze: | OpenAIRE |
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