Mechanisms involved in the antinociception induced by spinal administration of inosine or guanine in mice
| Autor: | Enderson Dias Alves de Oliveira, Cristhine Schallenberger, Marcos Dias Pinto da Silva, Elaine Elisabetsky, Ana Elisa Böhmer, Diogo O. Souza, Gisele Hansel, André Schmidt, Aécio C. Fagundes, Luis Valmor Cruz Portela, Lisiane O. Porciúncula, Michael Milman, Jean Pierre Oses |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Purine Male Nociception Guanine Pain AMPA receptor Pharmacology 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine medicine Animals Purine metabolism Inosine Injections Spinal Spinal cord Analgesics Chemistry Receptors Purinergic P1 030104 developmental biology Biochemistry Hypoxanthine-guanine phosphoribosyltransferase Purines NMDA receptor 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | European journal of pharmacology. 772 |
| ISSN: | 1879-0712 |
| Popis: | It is well known that adenine-based purines exert multiple effects on pain transmission. Recently, we have demonstrated that guanine-based purines may produce some antinociceptive effects against chemical and thermal pain in mice. The present study was designed to investigate the antinociceptive effects of intrathecal (i.t.) administration of inosine or guanine in mice. Additionally, investigation into the mechanisms of action of these purines, their general toxicity and measurements of CSF purine levels were performed. Animals received an i.t. injection of vehicle (30mN NaOH), inosine or guanine (up to 600nmol) and submitted to several pain models and behavioural paradigms. Guanine and inosine produced dose-dependent antinociceptive effects in the tail-flick, hot-plate, intraplantar (i.pl.) glutamate, i.pl. capsaicin and acetic acid pain models. Additionally, i.t. inosine inhibited the biting behaviour induced by spinal injection of capsaicin and i.t. guanine reduced the biting behaviour induced by spinal injection of glutamate or AMPA. Intrathecal administration of inosine (200nmol) induced an approximately 115-fold increase on CSF inosine levels. This study provides new evidence on the mechanism of action of extracellular guanine and inosine presenting antinociceptive effects following spinal administration. These effects seem to be related, at least partially, to the modulation of A1 adenosine receptors. |
| Databáze: | OpenAIRE |
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