Basophils Produce IL-4 and Accumulate in Tissues after Infection with a Th2-inducing Parasite
| Autor: | Fred D. Finkelman, Ann M. Dvorak, William E. Paul, Jinfang Zhu, Melanie Prout, Joseph F. Urban, Dragana Jankovic, Booki Min, Ellen S. Morgan, Graham LeGros, Jane Hu-Li |
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| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
green fluorescent protein
CD4-Positive T-Lymphocytes Immunology Green Fluorescent Proteins T cells Spleen chemical and pharmacologic phenomena Mice Transgenic Basophil Immunoglobulin E liver Article Green fluorescent protein 03 medical and health sciences Mice 0302 clinical medicine Th2 Cells parasitic diseases medicine cytokine Immunology and Allergy Animals Nippostrongylus brasiliensis Lung Interleukin 4 030304 developmental biology DNA Primers 0303 health sciences biology Trichostrongyloidea Reverse Transcriptase Polymerase Chain Reaction Tissue migration hemic and immune systems biology.organism_classification Flow Cytometry Molecular biology CD4 3. Good health Basophils Luminescent Proteins Microscopy Electron medicine.anatomical_structure Gene Expression Regulation biology.protein Interleukin-4 Antibody 030215 immunology |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 1540-9538 0022-1007 |
| Popis: | Using mice in which the eGfp gene replaced the first exon of the Il4 gene (G4 mice), we examined production of interleukin (IL)-4 during infection by the intestinal nematode Nippostrongylus brasiliensis (Nb). Nb infection induced green fluorescent protein (GFP)pos cells that were FcεRIpos, CD49bbright, c-kitneg, and Gr1neg. These cells had lobulated nuclei and granules characteristic of basophils. They were found mainly in the liver and lung, to a lesser degree in the spleen, but not in the lymph nodes. Although some liver basophils from naive mice express GFP, Nb infection enhanced GFP expression and increased the number of tissue basophils. Similar basophil GFP expression was found in infected Stat6−/− mice. Basophils did not increase in number in infected Rag2−/− mice; Rag2−/− mice reconstituted with CD4 T cells allowed significant basophil accumulation, indicating that CD4 T cells can direct both tissue migration of basophils and enhanced IL-4 production. IL-4 production was immunoglobulin independent and only partially dependent on IL-3. Thus, infection with a parasite that induces a “Th2-type response” resulted in accumulation of tissue basophils, and these cells, stimulated by a non-FcR cross-linking mechanism, are a principal source of in vivo IL-4 production. |
| Databáze: | OpenAIRE |
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