OLEUROPEIN
| Autor: | Evangelos J. Giamarellos-Bourboulis, Vassilios Koussoulas, Labros Sabracos, Hariklia Kranidioti, Ira Tzepi, John Vassiliadis, Pantelis Koutoukas, Thomas Tsaganos, Emilia Pelekanou, Vassiliki Karagianni, Helen Giamarellou, Ioannis Alexandrou, Michael Chrisofos, Taxiarchis Geladopoulos |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Survival Iridoid Glucosides Biology Critical Care and Intensive Care Medicine medicine.disease_cause Antioxidants Proinflammatory cytokine Microbiology Sepsis chemistry.chemical_compound Oleuropein Intensive care medicine Animals Humans Immunologic Factors Iridoids Pseudomonas Infections Amikacin Cells Cultured Pyrans Pseudomonas aeruginosa Monocyte medicine.disease Malondialdehyde Anti-Bacterial Agents medicine.anatomical_structure chemistry Emergency Medicine Rabbits medicine.drug |
| Zdroj: | Shock. 26:410-416 |
| ISSN: | 1073-2322 |
| DOI: | 10.1097/01.shk.0000226342.70904.06 |
| Popis: | Oleuropein, a novel immunomodulator derived from olive tree, was assessed in vitro and in experimental sepsis by Pseudomonas aeruginosa. After addition in monocyte and neutrophil cultures, malondialdehyde, TNF-!, IL-6, and bacterial counts were estimated in supernatants. Acute pyelonephritis was induced in 70 rabbits after inoculation of pathogen in the renal pelvis. Intravenous therapy was administered in four groups postchallenge by one multidrug- resistant isolate (A, controls; B, oleuropein; C, amikacin; D, both agents) and in three groups postchallenge by one susceptible isolate (E, controls; F, oleuropein; G, amikacin). Survival was recorded; bacterial growth in blood and organs was counted; endotoxins (LPS), malondialdehyde, total antioxidant status, and TNF-! in serum were estimated. TNF-! and IL-6 of cell supernatants were not increased compared with controls when triggered by LPS and P. aeruginosa. Counts of multidrug-resistant P. aeruginosa were decreased in monocyte supernatants. Median survival of groups A, B, C, D, E, F, and G were 3.00, 6.00, 2.00, 10.00, 1.00, 5.00, and 1.00 days, respectively. Bacteria in blood were lower at 48 h in groups B and D compared with A and in groups F and G compared with E. Total antioxidant status decreased steadily over time in groups A, C, D, and G, but not in groups B and F. TNF-! of groups B, C, and D was lower than A at 48 h. Tissue bacteria decreased in group F compared with E. Oleuropein prolonged survival in experimental sepsis probably by promoting phagocytosis or inhibiting biosynthesis of proinflammatory cytokines. KEYWORDS—Sepsis, Pseudomonas, antioxidants, immunomodulation |
| Databáze: | OpenAIRE |
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