Negative regulation of transcription by the type II arginine methyltransferase PRMT5

Autor: Alphonse Le Cam, Claude Sardet, Jin-Hyung Lee, Conception Paul, Vincent Negre, Jolanta Polanowska, Eric Fabbrizio, Sidney Pestka, Jeffry R. Cook, Selma El Messaoudi, M. Rousset
Přispěvatelé: Centre de génétique et de physiologie moléculaire et cellulaire (CGPhiMC), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Institut de Génétique Moléculaire de Montpellier (IGMM), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Génétique Végétale (GV), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Institut National Agronomique Paris-Grignon (INA P-G)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)
Rok vydání: 2002
Předmět:
Protein-Arginine N-Methyltransferases
Transcription
Genetic
Macromolecular Substances
Repressor
Biology
Arginine
Biochemistry
Histone H4
Mice
Xenopus laevis
03 medical and health sciences
0302 clinical medicine
Genes
Reporter
Histone arginine methylation
Transcription (biology)
Catalytic Domain
Cyclin E
bipartite repressor element e gene-expression saccharomyces-cerevisiae retinoblastoma protein human homolog cell-cycle histone h3 methylation kinase deacetylase
Genetics
Animals
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Protein Methyltransferases
Promoter Regions
Genetic
E2F
Molecular Biology
030304 developmental biology
0303 health sciences
Protein arginine methyltransferase 5
Scientific Reports
Promoter
3T3 Cells
Molecular biology
Chromatin
Rats
Repressor Proteins
Cyclin E1
Gene Expression Regulation
Liver
030220 oncology & carcinogenesis
Mutagenesis
Site-Directed
Oocytes
Zdroj: EMBO Reports
EMBO Reports, EMBO Press, 2002, 3 (7), pp.641--645
EMBO Reports, EMBO Press, 2002, 3 (7), pp.641--645. ⟨10.1093/embo-reports/kvf136⟩
ISSN: 1469-3178
1469-221X
DOI: 10.1093/embo-reports/kvf136
Popis: We have identified previously a repressor element in the transcription start site region of the cyclin E1 promoter that periodically associates with an atypical, high molecular weight E2F complex, termed CERC. Purification of native CERC reveals the presence of the type II arginine methyltransferase PRMT5, which can mono- or symetrically dimethylate arginine residues in proteins. Chromatin immunoprecipitations (ChIPs) show that PRMT5 is associated specifically with the transcription start site region of the cyclin El promoter. ChIP analyses also show that this correlates with the presence on the same promoter region of arginine-methylated proteins including histone H4, an in vitro substrate of PRMT5. Consistent with its presence within the repressor complex, forced expression of PRMT5 negatively affects cyclin E1 promoter activity and cellular proliferation, effects that require its methyltransferase activity. These data provide the first direct experimental evidence that a type II arginine methylase is involved in the control of transcription and proliferation.
Databáze: OpenAIRE
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