Suppression of the tert-butylhydroquinone toxicity by its grafting onto chitosan and further cross-linking to agavin toward a novel antioxidant and prebiotic material
| Autor: | Jorge Nieto-Sotelo, Rigoberto Hernández-Castro, Isabel Gracia-Mora, Miguel A. Hernández-Valdepeña, José Pedraza-Chaverri, Francisco Sánchez-Bartéz, Carmina Montiel, Keiko Shirai, Miquel Gimeno |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Antioxidant
tert-Butylhydroquinone Oxygen radical absorbance capacity medicine.medical_treatment Inulin 02 engineering and technology 010402 general chemistry 01 natural sciences Antioxidants Analytical Chemistry Chitosan Mice chemistry.chemical_compound Agave medicine Animals Organic chemistry ABTS Prebiotic General Medicine 021001 nanoscience & nanotechnology Hydroquinones 0104 chemical sciences Lactic acid Prebiotics chemistry 0210 nano-technology Food Science |
| Zdroj: | Food Chemistry. 199:485-491 |
| ISSN: | 0308-8146 |
| Popis: | The enzyme-mediated grafting of tert-butylhydroquinone (TBHQ) onto chitosan and further crosslinking to agave inulin (agavin) has been successfully achieved in a mild and non-toxic two-step route. The resulting products were characterized by nuclear magnetic resonance (NMR) and Infra-red spectroscopies to assess the molecular structure. The study of acute oral toxicity in mice revealed no adverse short-term effects of consumption in the synthesized materials with non-toxicity evidence until 2000 mg/kg through an oral acute administration. Importantly, this study proves that the compound maintains the radical scavenging capacity of the phenolic antioxidant upon ferric-reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC) assays with a measured half-maximal inhibitory concentration (IC50) for the best case of 1.54 g/L based on inhibition of 2,2'-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid diammonium salt (ABTS). Additionally, the novel compound presented high prebiotic activities as ascertained in the presence of lactic acid bacteria (LAB). |
| Databáze: | OpenAIRE |
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