Stromal vascular fraction promotes fibroblast migration and cellular viability in a hyperglycemic microenvironment through up-regulation of wound healing cytokines
| Autor: | Samuel Sam Wah Tay, Xin Yi Yeo, Zhen Chang Liang, Shaun S Tan, Sunil Sethi |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Clinical Biochemistry Population Cell Adipose tissue Pathology and Forensic Medicine Fibroblast migration Mice 03 medical and health sciences Cell Movement medicine Animals MTT assay education Molecular Biology Cells Cultured Cell Proliferation Wound Healing education.field_of_study Chemistry Fibroblasts Stromal vascular fraction Coculture Techniques Up-Regulation Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Adipose Tissue Hyperglycemia Cancer research Cytokines Stromal Cells Stem cell Wound healing |
| Zdroj: | Experimental and Molecular Pathology. 104:250-255 |
| ISSN: | 0014-4800 |
| Popis: | Diabetic wounds have impaired healing and a propensity for further morbidity, which may result in amputations. Stromal vascular fraction (SVF) is an autologous source of heterogeneous cell population obtained from adipose tissue, which is rich in stem cells and presents little immunogenicity to the host. In this study, we hypothesized that murine fibroblasts subjected to hyperglycemic conditions co-treated with SVF exhibit greater functional activity through the colorimetric MTT assay and a cell-monolayer in-vitro scratch assay. We sought to establish the underlying mechanism of action via the utility of an ELISA chemiluminescence array on the supernatant medium of the cells. Our results demonstrate that the mean percentage gap closure at 24 h in the hyperglycemia + SVF group was significantly greater at 41.1% ± 1.6% compared to the hyperglycemia alone group 16.6% ± 1.5% (post-hoc Bonferroni test p 0.001, n = 3) although there was no difference between the SVF and normoglycemia group. Further, this SVF group exhibited a significantly greater 2.4 fold increase in fibroblastic cell viability as compared to the hyperglycemia alone group (p = 0.001, n = 3). The supernatant medium of the cells upon testing with ELISA indicated that early phase wound healing cytokines including platelet-derived growth factor (p = 0.012, n = 3), interleukin-1 (p = 0.003, n = 3), basic fibroblast growth factor (p = 0.003, n = 3) and interleukin-10 (p = 0.009, n = 3) were expressed in significantly greater relative luminescent units in SVF as compared to hyperglycemia alone groups (Student t-test). Taken together and for the first time, our study shows that SVF is a promising therapeutic agent for up-regulating fibroblastic activity in a hyperglycemic microenvironment, and this result can be explained in part by the stimulation of wound-healing cytokines. |
| Databáze: | OpenAIRE |
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