Limitations of Cross-Talk Between Osteosarcoma and Bone Marrow-Derived Mesenchymal Stem Cells
| Autor: | Ezgi Daskin, Yasemin Basbinar, Ahu Pakdemirli, Caner Karaca, Turkan Yigitbasi, Hanife Ecenur Meco, Hülya Ellidokuz, Tuğba Uysal |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Volume: 3, Issue: 2 83-88 Journal of Basic and Clinical Health Sciences |
| ISSN: | 2564-7288 |
| Popis: | Objectives: Metastasis is a multi-step process which leads the tumor cells to escape from primer tumor region due to their need to gain malign phenotypes. While the effect of bone marrow-derived mesenchymal stem cells upon metastasis is not certain, some studies point out bone marrow-derived mesenchymal stem cells (BM-MSCs) to have this ability due to cell-cell interaction, released cytokines, and organization with the extracellular matrix in the micro-environment. Cross-talk via soluble factors also shifts the metastatic character. Patients and Methods: In this study, the effects of mesenchymal stem cells on tumor behavior by creating different microenvironments in 3-dimensional (3D) in vitro cancer model is analyzed. The BM-MSCs and osteosarcoma cells were co-cultured via hanging-drop modeled 3D structure in normoxic and hypoxic conditions, and the cross-talk was modeled to measure their chemoattractant effects The invasion and migration rates were measured with xCELLigence DP real-time cell analysis system. Mann Whitney U Test was used to compare independent samples. All P-values |
| Databáze: | OpenAIRE |
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